The Expression of G1 Cycins and Rb-E2F, and the Effect of Vitamin E on Hepatic Stellate Cells Activated by CCl4
사염화탄소에 의해 활성화된 간성상세포에서 G1 Cyclin 및 Rb-E2F 의 발현과 비타민 E 의 효과
이, 광재; 이, 관식; 한, 광협; 전, 재윤; 문, 영명; 오, 상환; 김, 호근
The Korean journal of gastroenterology, 38(4):262-269, 2001
The Korean journal of gastroenterology; Taehan Sohwagi Hakhoe chi
Background/Aims: It is obscure when the activation of hepatic stellate cells and the expression of its related factors occur in acute liver injury. Vitamin E is expected to prevent hepatic fibrosis. The aims of this study were
to establish the model of hepatic stellate cell activation in acute liver injury and to confirm the effect of vitamin E for preventing hepatic fibrosis. Methods: Male Sprague-Dawley rats were classified into two groups. The
one group received a single injection of CCl4 and the other group received injection of vitamin E daily and a single injection of CCl4. The serial changes of serum ALT, and [3H]thymidine uptake, α-SMA, cyclin D1,
cyclin E, CDK2, Rb, E2F-1 and NF-κB of stellate cells were measured. Results: The
serial changes of serum ALT levels, [3H]thymidine uptake, and α-SMA positive cells showed maximum increase at 32 hours after CCl4 injection. However, they were significantly decreased with injection of vitamin E. CDK4,
and CDK2 showed definite band at 16, 32, 48 hours after CCl4 injection, which
diminished or disappeared with injection of vitamin E. Cyclin D1, Rb, E2F-1 and NF-κB showed definite band at 32 hours after CCl4 injection, which also diminished or disappeared with injection of vitamin E. Conclusions: We
an in vivo model of hepatic stellate cell activation in acute liver injury and confirmed the effect of vitamin E in preventing hepatic fibrosis.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
Total Visit :2,913,675
Total Download :1,177,275
Today View :929
Ajou University Medical Information & Media Center 164 Worldcup-ro Yeongtong-gu Suwon 16499 Korea / TEL : 031-219-5312 / FAX : 031-219-5314 Copyright (c) Ajou University Medical Information & Media Center All Rights Reserved. AJOU Open Repository는 국립중앙도서관 OAK 보급사업으로 구축되었습니다.