Comparison of the Screening Media and Inoculation Methods in Detection of Vancomycin-Resistant Enterococci
Vancomycin 내성 장구균 검출을 위한 검색배지 및 접종방법의 비교
백, 세연; 이, 위교; 곽, 연식
Imsang pyŏngri wa chŏngdo kwanri, 20(1):187-192, 1998
Imsang pyŏngri wa chŏngdo kwanri; Journal of clinical pathology and quality control; 임상병리와 정도관리
Background : Vancomycin-resistant enterococci (VRE) became one of the clinically important causative organisms of the nosocomial infection lately, however, detection of VRE is still troublesome. This study was conducted to find the most effective screening media and inoculation method for detection of VRE.
Method : Twenty-nine strains of VRE (vanA : 5, vanB : 13, vanC : 11) that had been isolated in our laboratory and 19 strains of vancomycin-susceptible enterococci
were used for this study. Vancomycin was added in concentrations of 4 μg/mL, 6 μ
g/mL, 8 μg/mL each to brain heart infusion (BHI) agar and Mueller-Hinton (MH) agar.
Each strain of organisms was inoculated as a drop using a cotton swab to each medium
with the turbidity of McFarland 0.5 standard. Other inoculations were applied by
micropipet, with the volume of 1 μL (105 CFU) and 10 μL (106 CFU), respectively. The growth was checked after 18, 24, and 48
hours of incubation at 35℃ and the sensitivity and specificity were calculated.
Result : BHI agar appeared to be the better screening medium than MH agar.
Because, the sensitivity of detecting vancomycin resistance on BHI and MH agar were 79. 3% to 1o0%, and 62.1% to 96.5%, respectively. In case 6 μg/mL vancomycin was
tested, use of micropipet inoculation with either 1 μL or 10 μL inoculum revealed
100% In both sensitivity and specificity. However, when using a cotton swab, the
sensitivity fell to 96.5% In case of 4 μg/mL vancomycin, the sensitivity was 100%, the
specificity was 94.7%.
Conclusion : This study indicated that the effective laboratory method for detecting
VRE could be the inoculation with micropipet using BHI agar added 6 μg of vancomycin per ㎖.
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