0 158

Cited 0 times in

Validation of measurement of horse dust mite-specific IgE antibodies in serum using enzyme-linked immunosorbent assam

Other Title
효소면역법을 이용한 혈청내 집먼지진드기-특이 IgE 항체 측정의 적정화
Authors
유, 탁; 남, 동호; 박, 해심
Citation
Chʿŏnsik mit alrerugi, 19(2):200-207, 1999
Journal Title
Chʿŏnsik mit alrerugi; Journal of asthma, allergy and clinical immunology; Korean journal of asthma, allergy and clinical immunology; 천식 및 알레르기
ISSN
1226-8739
Abstract
Background : Measurement of allergen-specific IgE antibodies using enzyme-linked

immunosorbent assay(ELISA) has been developed and the results were shown to

correlate well with those obtained by radioimmunoassay(RIA). However, consensus on

the optimal condition and data expression method for the measurement of

allergen-specific IgE using ELISA is still not present.

Objective : To define the optimal condition for the measurement of allergen-specific

IgE using ELISA and to evaluate the accuracy and reproducibility of the results.

Method : We measured the concentrations of house dust mite-specific IgE antibodies

in serum samples by ELISA and RIA method (AlaSTAT, DPC, USA) using

standardized Dermatophagoides farinae antigen(kindly donated by Allergopharma Joachim

Ganzer KG, Reinbek, Germany).

Results : Optimal antigen coating amount was 2 ㎍/well and optimal serum dilution

was 1:10 for ELISA. The expression of the absolute concentration of house dust

mite-specific IgE antibodies within the unknown sample using serial dilutions of samples

and standard serum seemed to be more reasonable than the expression of absorbance

value at a single serum dilution, because the former method provided better inter-assay

variation and correlation with RIA results. The results of specific IgE measurement

using ELISA significantly correlated with RIA results(r=0.96, p<0.001, n=26).

Conclusion : These findings suggest that the measurement of allergen-specific IgE

antibodies using the ELISA method can be accurate and reliable if optimal assay

conditions and standardized data expression are applied.
Keywords
specific IgEhouse dust miteELISA
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Allergy
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse