The Effect of All-trans and 13-cis-retinoic Acid in Medulloblastoma and Glioblastoma Cell Culture
수아세포종 세포와 교모세포종 세포의 배양에서 all-trans와 13-cis retinoic acid의 세포증식억제 효과
윤, 수한; 김, 세혁; 안, 영환; 안, 영민; 조, 기홍; 조, 경기; 김, 성환
Taehan Soa Singyŏng Hakoe chi, 5(2):217-227, 1997
Taehan Soa Singyŏng Hakoe chi; Journal of Korea Child Neurology Association; 대한소아신경학회지
Purpose : It has been reported that retinoic acid, one of the most popular agents for
chemoprevention could inhibit the proliferation of many cancer cells including
neuroblastoma and glioblastoma. However, there is increasing demand reaccessing its in vitro inhibitory effect on the tumor proliferation because of the poor results from recent clinical trials of retinoic acid in the malignant brain tumor. Retinoic acid ptomoted the differentiation and apoptosis of tumor cell so that its effect might be obvious in the pediatric brain tumor. Therefore we are going to confirm the effectiveness of retinoic acid to inhibit the proliferation of the tumor cells; glioblastoma and medulloblastoma in
Methods : Medulloblastoma cells were derived from the primary culture of the patient's specimen, and glioblastoma cells were cell lines of 373-MG and 87-MG. We estimated growth inhibition rate of each tumor cells using MTT assay in the concentration from 10-12M to 10-5M of all-trans and 13-cis
Results : 13-cis retinoic acid in the concentration of 10-6M inhibited cell growth rate 10-22% on the 4th day of incubation, 10% on the 7th day, and 0-12% on the 14th day in the concentration from 10-6M to 10-5M.
All-trans retinoic acid inhibited cell growth rate less than 5% in the concentration less than 10-5M though the whole incubation period, but 42% on the 4th day, 37% on the 7th day, and 0% on the 14th day in the concentration of 10-5M. 13-cis retinoic acid inhibited cell growth rate 30% on the 4th day, 20% on the 7th and 14th day in the concentration between 10-6M and
10-5. All trans retinoic acid inhibited cell growth rate lessthan 5% on the 4th and 7th day. Medulloblastoma cells showed growth inhibition more than 25% by all-trans and 13-cis retinoic acid in the concentration less than 10-5M.
13-cis retinoic acid showed 25% growth inhibition in the concentration above
10-6M, but all-trans retinoic acid showed 40% growth inhibition in the same concentration.
Conclusion : We could not find the effect of retinoic acid in the glioblastoma cells due
to variable responses of the tumor cell growth inhibition in the concentration of
maximum tolerable dose. However, there is a significant inhibitory effect of
medulloblastoma cell proliferation both in the 13-cis and all-trans retinoic acid.
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