88 212

Cited 0 times in

Effect of Intravenous Immuoglobulin on Interleukin-6 Production in Human Skeletal Muscle Cell Line (HM4)

Other Title
인간 골격근세포주(HM4)에서 Interleukin-6 생성에 대한 정맥내 글로불린의 효과
주, 인수; 주, 억식; 김, 성회; 이, 용범
Taehan Sin'gyŏngkwa Hakhoe chi : sin'gyŏnghak, 22(3):249-254, 2004
Journal Title
Taehan Sin'gyŏngkwa Hakhoe chi : sin'gyŏnghak; Journal of the Korean Neurological Association : neurology
Background: Intravenous immunoglobulin (IVIg) has been widely used in the management of patients with various autoimmune neurological diseases, however, its action mechanisms have not fully been elucidated yet. This study focused on the effects of IVIg on the production of interleukin-6 (IL-6), one of major proinflammatory cytokine, using a human skeletal muscle cell line (HM4).

Methods: After HM4 cells were cultured in Dulbecco´s modified eagle´s medium (DMEM) containing 5% fetal bovine serum for 24 h, the culture medium was changed with serum-free media. TNF-α (tumor necrosis factor-α, 100 ng/mL) and IVIg (5 mg/mL) were treated alone or in combination and cultured for various time. RT-PCR and ELISA kit were employed for mRNA expression and secretion of IL-6, respectively.

Results: Treatment with TNF-α or/and IVIg significantly induced IL-6 mRNA expression (p<0.001). Although IL-6 production was markedly increased by TNF-α (p<0.001), IVIg treatment alone or in combination with TNF-α had no effect on the production of IL-6 except at 6 h after the treatment.

Conclusions: IVIg seems not to have a significant effect on IL-6 production as an action mechanism of its immunomodulatory capabilities, at least in the HM4 cell line.
Intravenous immunoglobulinIL-6Human skeletal muscle cell line
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Neurology
Files in This Item:
RIS (EndNote)
XLS (Excel)


해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.