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A Study on the Cross-allergenicity between Buckwheat and Rice Flour Using IgE-immunoblot Inhibition and ELISA-inhibition Test
|dc.description.abstract||Purpose : Buckwheat is one of the potent allergenic foods, whereas rice is considered
as the safest and hypoallergenic food in Korea. However rice can be a causative food
allergen, and several report about allergic reactions to rice in Japan. And it is partially
true that there is some cross-allergenicity among cereal allergens such as buckwheat,
barley, wheat, rice. So, we should consider a false positive reaction to rice during
allergic skin tests, especially in cereal allergic patients. We found that a part of
buckwheat allergic children showed positive skin reactions to rice antigens, without any
allergic reactions after rice ingestion. Therefore the positive skin test results to rice
were considered as the false positive reactions due to cross-allergenicity with buckwheat
allergen. To evaluate this possibility, we undertook this study.
Methods : To evaluate the cross-allergenicity between rice and buckwheat antigens.
With the buckwheat sensitive sera and crude extracts of buckwheat flour and rice flour
obtained in Korea, we carried out SDS-PAGE analysis, IgE immunoblot, Ige-immouoblot
inhibition test and ELISA ingibition test.
Results : Buckwheat extract showed more than 13 antigenic bands and rice extract
showed more than 9 bands by SDS-PAGE analysis. IgE immunoblot revealed at least
20 IgE-binding components(ranged 120-4 kDa) with buckwheat extract. Ige-binding
capacity of 22 kDa buckwheat component was markedly inhibited by rice extract and 8
kDa component was inhibited slightly. Using IgE ELISA inhibition test, we confirmed
buckwheat IgE-ELISA was profoundly inhibited by rice extract, and 50% inhibition was
shown in 10-100 ug/ml concentration of rice extract.
Conclusion : Taken together, the positive reactions to rice antigen would be shown by
the result of cross-allergenicity of rice with buckwheat antigen.
|dc.title||A Study on the Cross-allergenicity between Buckwheat and Rice Flour Using IgE-immunoblot Inhibition and ELISA-inhibition Test||-|
|dc.title.alternative||IgE-immunoblot Inhibition과 ELISA-inhibition Test를 이용한 메밀과 쌀 항원의 교차항원성에 관한 연구||-|
|dc.citation.title||Soa Allerŭgi mit Hohŭpgi Hakhoe chi||-|
|dc.citation.title||Pediatric allergy and respiratory disease||-|
|dc.identifier.bibliographicCitation||Soa Allerŭgi mit Hohŭpgi Hakhoe chi, 10(2):161-170, 2000||-|
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