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Immunoblot Analysis of Allergens in Mosquito, Culex pipiens

Other Title
우리나라에 서식하고 있는 모기(Culex pipiens)의 항원 추출과 항원성 분석
Authors
염, 혜영; 김, 규언; 류, 정우; 정, 병주; 이, 기영; 전, 성후; 용, 태순; 이, 한일; 이, 기선; 이, 수영
Citation
Soa Allerŭgi mit Hohŭpgi Hakhoe chi, 9(3):252-258, 1999
Journal Title
Soa Allerŭgi mit Hohŭpgi Hakhoe chi; Pediatric allergy and respiratory disease; 소아알레르기호흡기학회지
ISSN
1225-679X
Abstract
Purpose : Adverse reactions to mosquito bites have been recognized for some time,

and these usually consist of large swellings, generalized urticaria, angioedema, and less

frequently asthma. There have been some efforts to define immunologic characteristics

of mosquito allergens throughout the world. But, in Korea, the immunologic analysis of

mosquito allergen have not been made.

Methods : Extracts of locally distributed female mosquito Culex pipiens, were prepared

from their heads and thoraxes. By sodium dodecylsulfate-polyacrylamide gel

electrophoresis, proteins of two extracts were isolated. Then their proein bands were

transferred to nitrocellulose membrane. Immunoblotting was performed with sera of

allergic patients to mosquito bites, then completed by antibodies to human IgE.

Results : Immunoblotting showed IgE binding proteins weighing 70 kD, 60 kD, 34 kD

in Culex pipiens.

Conclusion : We found that mosquito antigens induced IgE response, and it suggested

that species-specific antigens exist. Further investigation using salivary gland extracts

from Culex genus are needed in identifying specific allergens.
Keywords
ImmunoblotMosquitoCulex pipiens
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pediatrics & Adolescent Medicine
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