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IgE Binding Pattern to Dermatophagoides Pteronyssinus in Younger and Older Asthmatic Children : An IgE Imnlunoblot Analysis

Other Title
질환을 앓고 있는 소아에서 Western-Blot을 이용한 D. pteronyssinus 조항원에 대한 IgE 항체 결합 양상 비교
Authors
이, 수영; 김, 수영; 홍, 창호; 홍, 천수
Citation
Soa Allerŭgi mit Hohŭpgi Hakhoe chi, 7(1):36-46, 1997
Journal Title
Soa Allerŭgi mit Hohŭpgi Hakhoe chi; Pediatric allergy and respiratory disease; 소아알레르기호흡기학회지
ISSN
1225-679X
Abstract
House dust mite is the most important causative allergens of childhood asthma and its

positive rate of allergy skin test reaches up to 80% in atopic asthmatic children in

Korea. Although the mite sensitization is more significant in older children(>4 years), it

is also important in some younger(<4 years) asthmatic children. To identify the major

allergens in Korean atopic asthmatic children and find the variations of IgE binding

pattern to Dermatophagoides pteronyssinus(Dp) in younger and older age groups, we

conducted IgE immunoblot.

Sera were collected in 25 children with Dp-sensitive atopic asthma : Group Ⅰ

(younger children aged 1-3), Goup Ⅱ(older children aged 4-12). Using our own-made

crude Dp extract, we conducted IgE- immunoblot (biotin-avidine-phosphatase system).

In the group Ⅰ, the 15KD protein was significantly bound by 69.2%(9/13) of sera and

98KD, 59KD, 31KD, 25KD components were bound by 8-16% of sera, respectively. In

the group Ⅱ, the 15KD and 25KD proteins were significantly bound Immunoblot by

100% and 67% of sera, and 18KD, 94KD by 33%, 98KD by 25%, 45KD by 8% of sera,

respectively.

In conclusion, the 15KD protein is the most prevalent IgE-binding antigen in all age

groups, on the other hands, IgE-sensitization to 25KD component is confirmed mainly in

the cases of older age group in this study.
Keywords
IgE Binding PatternDermatophagoides pteronyssinusIgE
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pediatrics & Adolescent Medicine
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