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Lithium Potentiates the FeCl₂ Induced Free Radical Injury in Primary Mouse Cortical Cell Culture

Other Title
대뇌 피질 신경세포 일차 배양에서 리튬의 FeCl₂에 의한 Free Radical Injury 증강 기전 연구
Authors
이, 승혜; 이, 영애; 변, 한열; 노, 재성
Citation
Taehan Chŏngsin Yangmul Hakhoe chi, 17(5):468-474, 2006
Journal Title
Taehan Chŏngsin Yangmul Hakhoe chi; The Korean journal of psychopharmacology; 대한정신약물학회지
ISSN
1017-57172092-5700
Abstract
Objectives : For the past half century, lithium has been used for the acute and prophylactic treatment of bipolar disorder and recurrent depression. Recently, new pharmacological effects of Li+ have appeared, showing that Li+ can influence neuronal injury. We tested the effects of Li+ on free radical induced neuronal injury in primary murine cortical cell cultures.



Methods : Cortical cells were prepared from fetal mice (embryonic day 15) and exposed to 30 μM Fe²+ alone or with 5 mM Li+ or 5 mM Li+ alone for 24 hrs at Days in vitro (DIV) 14. Neuronal death was analyzed by measuring lactate dehydrogenase (LDH) release into media. The fluorescence of 2’,7’-dichlorofluorescin (DCF) was measured in as a mean of estimating the formation of reactive oxygen species (ROS).



Results : Li+ alone does not produce neuronal injury itself but it potentiates Fe²+-induced neuronal injury through increasing the production of free radical. Conclusion : This study suggests that the effects of Li+ on neuronal survivorship may be injury type dependent and Li+ potentiate the free radical injury. Therefore in practice clinician should be cautious in using the lithium in the treatment of brain injured patients.
Keywords
LithiumFree radical injuryNecrosis
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Psychiatry & Behavioural Sciences
Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
AJOU Authors
정, 영기노, 재성곽, 병주
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