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The ERK-RSK1 activation by growth factors at G2 phase delays cell cycle progression and reduces mitotic aberrations.

Nam, HJ; Kim, S; Lee, MW; Lee, BS; Hara, T; Saya, H; Cho, H; Lee, JH
Cellular signalling, 20(7):1349-1358, 2008
Journal Title
Cellular signalling
Growth factors accelerate G0 to S progression in the cell cycle, however, the roles of growth factors in other cell cycle phases are largely unknown. Here, we show that treatment of HeLa cells with hepatocyte growth factor (HGF) at G2 phase induced the G2/M transition delay as evidenced by FACS analysis as well as by mitotic index and time-lapse analyses. Growth factors such as epidermal growth factor (EGF) and fibroblast growth factor (FGF) also induced G2/M transition delay like HGF. HGF treatment at G2 phase causes a delayed activation of cyclin B1-associated kinase and a diminished nuclear translocation of cyclin B1. Either U0126, a MAPK kinase (MEK) inhibitor, or kinase-dead mutant of ribosomal S6 kinase (RSK) abolished the delay. Additionally, knockdown of RSK1, but not RSK2, with siRNA abrogated the delay, indicating that the extracellular-regulated protein kinase (ERK)-RSK1 mediates the HGF-induced delay. We further found that the delay in G2/M transition of cells expressing oncogenic HGF receptor, M1268T, was abolished by RSK1 knockdown. Intriguingly, we observed that HGF induced chromosomal segregation defects, and depletion of RSK1, but not RSK2, aggravated these chromosomal aberrations. Taken together, the ERK-RSK1 activation by growth factors delays G2/M transition and this might be required to maintain genomic integrity during growth factor stimulation.
MeSH terms
CDC2 Protein Kinase/metabolismCell Cycle Proteins/metabolismChromosome Segregation/drug effectsCyclin B/metabolismCyclin B1DNA-Binding Proteins/metabolismEnzyme Activation/drug effectsExtracellular Signal-Regulated MAP Kinases/metabolism*G2 Phase/drug effects*Hela CellsHumansIntercellular Signaling Peptides and Proteins/pharmacology*Mitosis/drug effects*Mutant Proteins/metabolismProtein-Serine-Threonine Kinases/metabolismProto-Oncogene Proteins c-met/metabolismRibosomal Protein S6 Kinases, 90-kDa/metabolism*Tumor Suppressor Proteins/metabolism
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Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
AJOU Authors
김, 수정조, 혜성이, 재호
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