7 229

Cited 62 times in

Uridine 5'-diphosphate induces chemokine expression in microglia and astrocytes through activation of the P2Y6 receptor

Authors
Kim, B; Jeong, HK; Kim, JH; Lee, SY; Jou, I; Joe, EH
Citation
Journal of immunology (Baltimore, Md. : 1950), 186(6):3701-3709, 2011
Journal Title
Journal of immunology (Baltimore, Md. : 1950)
ISSN
0022-17671550-6606
Abstract
Chemokines play critical roles in inflammation by recruiting inflammatory cells to injury sites. In this study, we found that UDP induced expression of chemokines CCL2 (MCP-1) and CCL3 (MIP-1α) in microglia, astrocytes, and slice cultures by activation of P2Y(6). Interestingly, CCL2 was more highly expressed than CCL3. However, CCL2 synthesis kinetics in response to UDP differed in microglia and astrocytes; microglia rapidly produced small amounts of CCL2, whereas astrocytes continuously synthesized large amounts of CCL2, resulting in a high ultimate level of the chemokine. UDP-induced chemokine expression was reduced in the presence of a specific antagonist of P2Y(6) (MRS2578) or small interfering RNA directed against the P2Y(6) gene. Inhibition of phospholipase C and calcium increase, downstream signaling pathways of Gq-coupled P2Y(6), reduced UDP-induced chemokine expression. UDP activated two calcium-activated transcription factors, NFATc1 and c2. Furthermore, inhibitors of calcineurin (a phosphatase activating NFAT) and NFAT reduced UDP-induced chemokine synthesis. We also found, using a transmigration assay, that UDP-treated astrocytes recruited monocytes. These results suggest that UDP induces chemokine expression in microglia and astrocytes of the injured brain by activation of P2Y(6) receptors.
MeSH terms
AnimalsAnimals, NewbornAstrocytes/*immunology/*metabolism/pathologyCell Movement/immunologyCells, CulturedCerebral Cortex/immunology/pathology/secretionChemokine CCL2/*biosynthesisChemokine CCL3/*biosynthesisMicroglia/*immunology/*metabolism/pathologyMonocytes/immunologyOrgan Culture TechniquesRatsRats, Sprague-DawleyReceptors, Purinergic P2/*metabolismUridine Diphosphate/*physiology/secretion
DOI
10.4049/jimmunol.1000212
PMID
21317391
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
Journal Papers > Research Organization > Chronic Inflammatory Disease Research Center
AJOU Authors
김, 범수이, 상윤주, 일로조, 은혜
Full Text Link
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse