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A Tat-grafted anti-nucleic acid antibody acquires nuclear-localization property and a preference for TAR RNA

Authors
Jeong, JG; Kim, DS; Kim, YS; Kwon, MH
Citation
Biochemical and biophysical research communications, 406(3):403-407, 2011
Journal Title
Biochemical and biophysical research communications
ISSN
0006-291X1090-2104
Abstract
The 3D8 single chain variable fragment (3D8 scFv) is an anti-nucleic acid antibody that can hydrolyze nucleic acids and enter the cytosol of cells without reaching the nucleus. The Tat peptide, derived from the basic region of the HIV-1 Tat protein, translocates to cell nuclei and has TAR RNA binding activity. In this study, we generated a Tat-grafted antibody ((H₃)Tat-3D8) by replacing complementarity-determining region 3 (CDR3) within the VH domain of the 3D8 scFv with a Tat₄₈₋₆₀ peptide (GRKKRRQRRRPPQ). (H₃)Tat-3D8 retained the DNA-binding and DNA-hydrolyzing activity of the scFv, and translocated to the nuclei of HeLa cells and preferentially recognized TAR RNA. Thus, the properties associated with the Tat peptide were transferred to the antibody via Tat-grafting without loss of the intrinsic DNA-binding and hydrolyzing activities of the 3D8 scFv antibody.
MeSH terms
Antibodies, Antinuclear/*immunology/metabolismBase SequenceCell Nucleus/*immunology/metabolismDNA/immunology*HIV-1HeLa CellsHumansHydrolysisMolecular Sequence DataProtein Structure, TertiaryRNA, Viral/*immunology/metabolismRNA-Binding Proteins/*immunology/metabolismSingle-Chain Antibodies/immunology/metabolism
DOI
10.1016/j.bbrc.2011.02.054
PMID
21329654
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
AJOU Authors
권, 명희
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