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Glycogen synthase kinase-3beta regulates etoposide-induced apoptosis via Bcl-2 mediated caspase-3 activation in C3H10T1/2 cells.
DC Field | Value | Language |
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dc.contributor.author | Yun, SI | - |
dc.contributor.author | Yoon, HY | - |
dc.contributor.author | Chung, YS | - |
dc.date.accessioned | 2010-12-17T02:15:47Z | - |
dc.date.available | 2010-12-17T02:15:47Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 1360-8185 | - |
dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/644 | - |
dc.description.abstract | Glycogen synthase kinase-3beta (GSK3beta) controls the survival of osteoblasts during bone development through Wnt canonical signaling. GSK3beta is a key factor for osteoblastogenesis, but relatively less is known regarding its role in osteoblast apoptosis. Genotoxic stress induced by etoposide promoted apoptotic signaling by GSK3beta activation in C3H10T1/2 cells, a mouse mesenchymal cell line. Etoposide led to the time-dependent activation of GSK3beta and caspase-3, which resulted in PARP cleavage. LiCl (a specific inhibitor) and siRNA (gene knock-down) of GSK3beta prevented the effects of etoposide on apoptosis. Staurosporine also induced apoptosis in C3H10T1/2 cells, but LiCl could not rescue. Bcl-2 was decreased in the cells by exposure to etoposide. LiCl completely recovered Bcl-2 expression as shown by both the mRNA and the protein expression levels. In conclusion, etoposide-induced apoptosis in C3H10T1/2 cells is mediated by GSK3beta, which leads to caspase-3 activation via decrease in Bcl-2 expression. | - |
dc.language.iso | en | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Apoptosis | - |
dc.subject.MESH | Caspase 3 | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Cytoprotection | - |
dc.subject.MESH | Enzyme Activation | - |
dc.subject.MESH | Etoposide | - |
dc.subject.MESH | Gene Expression Regulation, Enzymologic | - |
dc.subject.MESH | Gene Silencing | - |
dc.subject.MESH | Glycogen Synthase Kinase 3 | - |
dc.subject.MESH | Lithium Chloride | - |
dc.subject.MESH | Mice | - |
dc.subject.MESH | Proto-Oncogene Proteins c-bcl-2 | - |
dc.subject.MESH | Staurosporine | - |
dc.title | Glycogen synthase kinase-3beta regulates etoposide-induced apoptosis via Bcl-2 mediated caspase-3 activation in C3H10T1/2 cells. | - |
dc.type | Article | - |
dc.identifier.pmid | 19408126 | - |
dc.contributor.affiliatedAuthor | 정, 윤석 | - |
dc.type.local | Journal Papers | - |
dc.identifier.doi | 10.1007/s10495-009-0348-4 | - |
dc.citation.title | Apoptosis : an international journal on programmed cell death | - |
dc.citation.volume | 14 | - |
dc.citation.number | 6 | - |
dc.citation.date | 2009 | - |
dc.citation.startPage | 771 | - |
dc.citation.endPage | 777 | - |
dc.identifier.bibliographicCitation | Apoptosis : an international journal on programmed cell death, 14(6). : 771-777, 2009 | - |
dc.identifier.eissn | 1573-675X | - |
dc.relation.journalid | J013608185 | - |
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