4 207

Cited 15 times in

CD4(+) CD25(+) regulatory T cells ameliorate Behcet's disease-like symptoms in a mouse model.

Authors
J, Shim; ES, Lee; S, Park; D, Bang; S, Sohn
Citation
Cytotherapy, 13(7):835-847, 2011
Journal Title
Cytotherapy
ISSN
1465-32491477-2566
Abstract
BACKGROUND AIMS: Behcet's disease (BD) is a chronic, multisystemic inflammatory disorder with arthritic, gastrointestinal, mucocutaneous, ocular, vascular and central nervous system involvement. It is well known that CD4(+) CD25(+) T-regulatory (Treg) cells prevent harmful immune responses to self- and non-self-antigens. In the present study, the role of Treg cells in herpes simplex virus (HSV)-induced BD-like symptoms was investigated.



METHODS: HSV type 1 (F strain) inoculation of the earlobe of ICR mice has been shown to induce the development of BD-like symptoms. To determine whether the effect of Treg was associated with change in BD-like symptoms, CD4(+) CD25(+) T cells from the splenocytes of normal mice were adoptively transferred intravenously. Treg cells of splenocytes were significantly elevated following the transfer of 3 × 10(5) CD4(+) CD25(+) T cells to BD-like mice compared with the control group.



RESULTS: The transfer of CD4(+) CD25(+) T cells to BD mice improved the symptoms, and the serum protein levels of interleukin (IL)-10, IL-6 and IL-17 were significantly altered compared with the control groups. Intravenous injection of anti-CD25 antibody to BD mice reduced the frequency of CD4(+) CD25(+) T cells and increased the BD severity score. We confirmed the influence of CD4(+) CD25(+) T cells on BD-like mice.



CONCLUSIONS: These results show that up-regulation of the CD4(+) CD25(+) T cells in BD-like mice improves the inflammatory symptoms, while down-regulation of CD25(+) T cells is associated with deteriorated symptoms. Furthermore, these findings are correlated with changes in pro-inflammatory and anti-inflammatory cytokine levels.
MeSH terms
Adoptive TransferAnimalsBehcet Syndrome/*immunology/metabolism/*therapy/virologyCD4-Positive T-Lymphocytes/*immunologyCells, CulturedDisease Models, AnimalForkhead Transcription Factors/immunology/metabolismHerpesvirus 1, Human/pathogenicityInterferon-gamma/bloodInterleukin-10/bloodInterleukin-17/bloodInterleukin-2 Receptor alpha Subunit/*immunologyInterleukin-6/bloodMaleMiceMice, Inbred ICRSpleen/cytology/immunologyT-Lymphocytes, Regulatory/*immunologyTransforming Growth Factor beta/bloodTumor Necrosis Factor-alpha/bloodUp-Regulation
DOI
10.3109/14653249.2011.571245
PMID
21506890
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Dermatology
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
AJOU Authors
이, 은소박, 선손, 성향
Full Text Link
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse