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CD4(+) CD25(+) regulatory T cells ameliorate Behcet's disease-like symptoms in a mouse model.

J, Shim; ES, Lee; S, Park; D, Bang; S, Sohn
Cytotherapy, 13(7):835-847, 2011
Journal Title
BACKGROUND AIMS: Behcet's disease (BD) is a chronic, multisystemic inflammatory disorder with arthritic, gastrointestinal, mucocutaneous, ocular, vascular and central nervous system involvement. It is well known that CD4(+) CD25(+) T-regulatory (Treg) cells prevent harmful immune responses to self- and non-self-antigens. In the present study, the role of Treg cells in herpes simplex virus (HSV)-induced BD-like symptoms was investigated.

METHODS: HSV type 1 (F strain) inoculation of the earlobe of ICR mice has been shown to induce the development of BD-like symptoms. To determine whether the effect of Treg was associated with change in BD-like symptoms, CD4(+) CD25(+) T cells from the splenocytes of normal mice were adoptively transferred intravenously. Treg cells of splenocytes were significantly elevated following the transfer of 3 × 10(5) CD4(+) CD25(+) T cells to BD-like mice compared with the control group.

RESULTS: The transfer of CD4(+) CD25(+) T cells to BD mice improved the symptoms, and the serum protein levels of interleukin (IL)-10, IL-6 and IL-17 were significantly altered compared with the control groups. Intravenous injection of anti-CD25 antibody to BD mice reduced the frequency of CD4(+) CD25(+) T cells and increased the BD severity score. We confirmed the influence of CD4(+) CD25(+) T cells on BD-like mice.

CONCLUSIONS: These results show that up-regulation of the CD4(+) CD25(+) T cells in BD-like mice improves the inflammatory symptoms, while down-regulation of CD25(+) T cells is associated with deteriorated symptoms. Furthermore, these findings are correlated with changes in pro-inflammatory and anti-inflammatory cytokine levels.
MeSH terms
Adoptive TransferAnimalsBehcet Syndrome/*immunology/metabolism/*therapy/virologyCD4-Positive T-Lymphocytes/*immunologyCells, CulturedDisease Models, AnimalForkhead Transcription Factors/immunology/metabolismHerpesvirus 1, Human/pathogenicityInterferon-gamma/bloodInterleukin-10/bloodInterleukin-17/bloodInterleukin-2 Receptor alpha Subunit/*immunologyInterleukin-6/bloodMaleMiceMice, Inbred ICRSpleen/cytology/immunologyT-Lymphocytes, Regulatory/*immunologyTransforming Growth Factor beta/bloodTumor Necrosis Factor-alpha/bloodUp-Regulation
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Dermatology
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
AJOU Authors
이, 은소박, 선손, 성향
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