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Poncirin promotes osteoblast differentiation but inhibits adipocyte differentiation in mesenchymal stem cells.

Yoon, HY; Yun, SI; Kim, BY; Jin, Q; Woo, ER; Jeong, SY; Chung, YS
European journal of pharmacology, 664(1-3):54-59, 2011
Journal Title
European journal of pharmacology
Poncirin, flavanone glycoside, isolated from the fruit of Poncirus trifoliata, has anti-bacterial and anti-inflammatory activities. In this study, the effects of poncirin on the differentiation of mesenchymal stem cells were investigated. The C3H10T1/2 mesenchymal stem cells and primary bone marrow mesenchymal stem cells were studied. In the C3H10T1/2 cells, poncirin prevented adipocyte differentiation, as demonstrated by inhibition of cytoplasm lipid droplet accumulation and peroxisome proliferator-activating receptor-γ (PPAR-γ) and CCAAT-enhancer-binding protein-β (C/EBP-β) mRNA expression. By contrast, poncirin enhanced the expression of the key osteogenic transcription factors, runt-related transcription factor 2 (Runx2) and transcriptional coactivator with PDZ-binding motif (TAZ). Poncirin also enhanced expression of the osteogenic marker genes including alkaline phosphatase (ALP) and osteocalcin (OC). Poncirin increased mineral nodule formation in primary bone marrow mesenchymal stem cells. These results suggest that poncirin prevents adipogenesis and enhances osteoblast differentiation in mesenchymal stem cells.
MeSH terms
Adipocytes/*cytology/*drug effectsAdipogenesis/drug effectsAnimalsBiological Markers/metabolismBone Marrow Cells/cytologyCell Differentiation/*drug effectsCell LineCore Binding Factor Alpha 1 Subunit/genetics/metabolismFemur/drug effects/metabolismFlavonoids/*pharmacologyGene Expression Regulation/drug effectsMaleMesenchymal Stem Cells/*cytology/drug effects/metabolismMiceMinerals/metabolismOsteoblasts/*cytology/*drug effectsTibia/drug effects/metabolismTranscription, Genetic/drug effects
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Endocrinology & Metabolism
Journal Papers > School of Medicine / Graduate School of Medicine > Medical Genetics
AJOU Authors
정, 선용정, 윤석
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