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Involvement of visfatin in palmitate-induced upregulation of inflammatory cytokines in hepatocytes.

DC Field Value Language
dc.contributor.authorChoi, YJ-
dc.contributor.authorChoi, SE-
dc.contributor.authorHa, ES-
dc.contributor.authorKang, Y-
dc.contributor.authorHan, SJ-
dc.contributor.authorKim, DJ-
dc.contributor.authorLee, KW-
dc.contributor.authorKim, HJ-
dc.date.accessioned2012-04-23T04:58:38Z-
dc.date.available2012-04-23T04:58:38Z-
dc.date.issued2011-
dc.identifier.issn0026-0495-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/6503-
dc.description.abstractFree fatty acids (FFAs) lead to the activation of inflammatory pathways related to the induction of insulin resistance. Visfatin is known to play a role in obesity-related metabolic diseases and inflammatory conditions. Here, the role of visfatin in FFA-induced inflammation was investigated in hepatocytes. The following factors were examined: (1) the protein and messenger RNA (mRNA) expression of visfatin in the liver tissue of insulin-resistant rats and in (2) in HepG2 cells treated with palmitate, (3) the palmitate-induced mRNA expression and protein synthesis of interleukin-6 and tumor necrosis factor-α in HepG2 cells transfected with visfatin-specific small interfering RNA, and (4) the expression of visfatin in HepG2 cells treated with a nuclear factor-κB (NF-κB) inhibitor (SN50) and infected with Ad-IκBα. The protein and mRNA levels of visfatin were significantly higher in insulin-resistant rat liver tissue compared with the control group. Visfatin expression and protein synthesis significantly increased in HepG2 cells treated with palmitate in a time- and concentration-dependent manner. Visfatin-specific small interfering RNA significantly decreased the palmitate-induced mRNA expression and protein synthesis of interleukin-6 and tumor necrosis factor-α. A NF-κB inhibitor induced the downregulation of visfatin in HepG2 cells following treatment with palmitate. HepG2 cells infected with Ad-IκBα showed decreased expression of visfatin following treatment with palmitate. The expression of visfatin is closely associated with the expression of proinflammatory cytokines in FFA-induced inflammation and is significantly decreased by NF-κB inhibition in HepG2 cells. Visfatin may play a role in FFA-induced inflammation in hepatocytes through the NF-κB pathway.-
dc.language.isoen-
dc.subject.MESHAdenoviridae Infections-
dc.subject.MESHAnimals-
dc.subject.MESHBlood Glucose/metabolism-
dc.subject.MESHCholesterol/blood-
dc.subject.MESHCytokines/*metabolism-
dc.subject.MESHEnzyme-Linked Immunosorbent Assay-
dc.subject.MESHHep G2 Cells-
dc.subject.MESHHepatocytes/*metabolism-
dc.subject.MESHHumans-
dc.subject.MESHI-kappa B Proteins/metabolism-
dc.subject.MESHImmunoblotting-
dc.subject.MESHInflammation/*metabolism-
dc.subject.MESHInsulin Resistance-
dc.subject.MESHInterleukin-6/blood-
dc.subject.MESHNicotinamide Phosphoribosyltransferase/genetics/*metabolism-
dc.subject.MESHObesity/*metabolism-
dc.subject.MESHPalmitic Acid/metabolism-
dc.subject.MESHPeptides/metabolism-
dc.subject.MESHRNA, Messenger/metabolism-
dc.subject.MESHRNA, Small Interfering/metabolism-
dc.subject.MESHRats-
dc.subject.MESHRats, Zucker-
dc.subject.MESHReal-Time Polymerase Chain Reaction-
dc.subject.MESHTriglycerides/blood-
dc.subject.MESHTumor Necrosis Factor-alpha/blood-
dc.subject.MESHUp-Regulation-
dc.titleInvolvement of visfatin in palmitate-induced upregulation of inflammatory cytokines in hepatocytes.-
dc.typeArticle-
dc.identifier.pmid21664630-
dc.identifier.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0026-0495(11)00128-4-
dc.contributor.affiliatedAuthor최, 용준-
dc.contributor.affiliatedAuthor강, 엽-
dc.contributor.affiliatedAuthor한, 승진-
dc.contributor.affiliatedAuthor김, 대중-
dc.contributor.affiliatedAuthor이, 관우-
dc.contributor.affiliatedAuthor김, 혜진-
dc.type.localJournal Papers-
dc.identifier.doi10.1016/j.metabol.2011.05.003-
dc.citation.titleMetabolism: clinical and experimental-
dc.citation.volume60-
dc.citation.number12-
dc.citation.date2011-
dc.citation.startPage1781-
dc.citation.endPage1789-
dc.identifier.bibliographicCitationMetabolism: clinical and experimental, 60(12):1781-1789, 2011-
dc.identifier.eissn1532-8600-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Endocrinology & Metabolism
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
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