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Effect of Toll-like receptor 4 gene polymorphisms on work-related respiratory symptoms and sensitization to wheat flour in bakery workers.

DC Field Value Language
dc.contributor.authorCho, HJ-
dc.contributor.authorKim, SH-
dc.contributor.authorKim, JH-
dc.contributor.authorChoi, H-
dc.contributor.authorSon, JK-
dc.contributor.authorHur, GY-
dc.contributor.authorPark, HS-
dc.date.accessioned2012-04-24T01:53:13Z-
dc.date.available2012-04-24T01:53:13Z-
dc.date.issued2011-
dc.identifier.issn1081-1206-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/6525-
dc.description.abstractBACKGROUND: Bakery workers are exposed to flour allergens and endotoxins, which interact to induce allergic responses and respiratory symptoms. We hypothesized that Toll-like receptor 4 (TLR4) may be involved in the development of work-related respiratory symptoms and sensitization to wheat flour.



OBJECTIVE: To investigate the genetic contribution of TLR4 to respiratory symptoms and sensitization to wheat flour in bakery workers, we performed a genetic association study of TLR4 in Korean bakery workers.



METHODS: A total of 381 workers completed a questionnaire regarding work-related symptoms. Skin prick tests with common and occupational allergens were done, and specific antibodies to wheat flour were measured by enzyme-linked immunosorbent assay. Two single-nucleotide polymorphisms (SNPs) of the TLR4 gene (-2027A>G and -1608T>C) were genotyped, and the functional effects of the polymorphisms were analyzed using the luciferase reporter and electrophoretic mobility shift assay.



RESULTS: Homozygotes for the -2027G and -1608C alleles exhibited a lower prevalence of work-related lower respiratory symptoms than carriers of the -2027AA/AG (P = .007) and -1608TT/TC (P =.021) genotypes. Furthermore, haplotype analysis indicated that workers with the haplotype 2, ht2 [GC], had fewer work-related lower respiratory symptoms (P = .021). The ht2 [GC] construct showed lower promoter activity than the haplotype 1, ht1[AT], in both BEAS-2B (P = .001) and U937 cells (P = .007).



CONCLUSION: Bakery workers carrying the TLR4 variants are at lower risk of developing work-related chest symptoms. This finding suggests that the TLR4 gene may be involved in allergic sensitization to wheat flour as well as endotoxin-induced respiratory symptoms in endotoxin-allergen-exposed workers and that carriers of TLR4 variants are less affected by environmental exposure.
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dc.language.isoen-
dc.subject.MESHAdult-
dc.subject.MESHAllergens/diagnostic use-
dc.subject.MESHAsian Continental Ancestry Group/genetics-
dc.subject.MESHAsthma/epidemiology/etiology/*genetics-
dc.subject.MESHFemale-
dc.subject.MESHFlour/*adverse effects-
dc.subject.MESHGenetic Association Studies-
dc.subject.MESHHaplotypes-
dc.subject.MESHHumans-
dc.subject.MESHMale-
dc.subject.MESHMiddle Aged-
dc.subject.MESHOccupational Diseases/epidemiology/etiology/*genetics-
dc.subject.MESHPolymorphism, Genetic-
dc.subject.MESHPrevalence-
dc.subject.MESHRhinitis/epidemiology-
dc.subject.MESHSkin Tests-
dc.subject.MESHToll-Like Receptor 4/*genetics-
dc.subject.MESHYoung Adult-
dc.titleEffect of Toll-like receptor 4 gene polymorphisms on work-related respiratory symptoms and sensitization to wheat flour in bakery workers.-
dc.typeArticle-
dc.identifier.pmid21704886-
dc.identifier.urlhttp://linkinghub.elsevier.com/retrieve/pii/S1081-1206(11)00256-0-
dc.contributor.affiliatedAuthor김, 승현-
dc.contributor.affiliatedAuthor박, 해심-
dc.type.localJournal Papers-
dc.identifier.doi10.1016/j.anai.2011.04.003-
dc.citation.titleAnnals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology-
dc.citation.volume107-
dc.citation.number1-
dc.citation.date2011-
dc.citation.startPage57-
dc.citation.endPage64-
dc.identifier.bibliographicCitationAnnals of allergy, asthma & immunology : official publication of the American College of Allergy, Asthma, & Immunology, 107(1):57-64, 2011-
dc.identifier.eissn1534-4436-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Allergy
Journal Papers > Research Organization > Regional Clinical Trial Center
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