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Withaferin A enhances radiation-induced apoptosis in Caki cells through induction of reactive oxygen species, Bcl-2 downregulation and Akt inhibition.

DC Field Value Language
dc.contributor.authorYang, ES-
dc.contributor.authorChoi, MJ-
dc.contributor.authorKim, JH-
dc.contributor.authorChoi, KS-
dc.contributor.authorKwon, TK-
dc.date.accessioned2012-04-24T04:30:40Z-
dc.date.available2012-04-24T04:30:40Z-
dc.date.issued2011-
dc.identifier.issn0009-2797-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/6532-
dc.description.abstractWithaferin A (Wit A), a natural compound derived from the medicinal plant Withania somnifera, has been reported for the anti-tumor effects, including the inhibition of tumor cell growth, metastasis and angiogenesis. In this study, we investigated the effect of Wit A on radiation-induced apoptosis in human renal cancer cells (Caki cells). Our results showed that, compared with Wit A or radiation alone, the combination of both resulted in a significant enhancement of apoptosis, showing the increase in the cleavage of caspase-3 and PARP as well as sub-G1 cell population. In addition, reactive oxygen species (ROS) generation was correlated with the enhancement of radiation-induced apoptosis by Wit A. Wit A downregulated Bcl-2 protein levels and ectopic expression of Bcl-2 in Caki cells attenuated the apoptosis induced by Wit A plus radiation. Taken together, these results indicate that Wit A enhanced radiation-induced apoptosis in Caki cells through ROS generation, down-regulation of Bcl-2 and Akt dephosphorylation. Thus, our study shows that Wit A may be used as an effective radiosensitizer in cancer therapy.-
dc.language.isoen-
dc.subject.MESHAntineoplastic Agents-
dc.subject.MESHApoptosis-
dc.subject.MESHCaspase 3-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHG1 Phase-
dc.subject.MESHHumans-
dc.subject.MESHPlants, Medicinal-
dc.subject.MESHPoly(ADP-ribose) Polymerases-
dc.subject.MESHProto-Oncogene Proteins c-akt-
dc.subject.MESHProto-Oncogene Proteins c-bcl-2-
dc.subject.MESHRadiation, Ionizing-
dc.subject.MESHReactive Oxygen Species-
dc.subject.MESHWithania-
dc.subject.MESHWithanolides-
dc.titleWithaferin A enhances radiation-induced apoptosis in Caki cells through induction of reactive oxygen species, Bcl-2 downregulation and Akt inhibition.-
dc.typeArticle-
dc.identifier.pmid21256832-
dc.identifier.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0009-2797(11)00020-2-
dc.contributor.affiliatedAuthor최, 경숙-
dc.type.localJournal Papers-
dc.identifier.doi10.1016/j.cbi.2011.01.015-
dc.citation.titleChemico-biological interactions-
dc.citation.volume190-
dc.citation.number1-
dc.citation.date2011-
dc.citation.startPage9-
dc.citation.endPage15-
dc.identifier.bibliographicCitationChemico-biological interactions, 190(1). : 9-15, 2011-
dc.identifier.eissn1872-7786-
dc.relation.journalidJ000092797-
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Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
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