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Combination of withaferin A and X-ray irradiation enhances apoptosis in U937 cells.

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dc.contributor.authorYang, ES-
dc.contributor.authorChoi, MJ-
dc.contributor.authorKim, JH-
dc.contributor.authorChoi, KS-
dc.contributor.authorKwon, TK-
dc.date.accessioned2012-04-24T05:38:16Z-
dc.date.available2012-04-24T05:38:16Z-
dc.date.issued2011-
dc.identifier.issn0887-2333-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/6537-
dc.description.abstractCombined treatment with radiation has improved the outcome in various cancers and many radiosensitizers are used to enhance the therapeutic efficiency of radiotherapy. Withaferin A (Wit A), a natural compound derived from the medicinal plant Withania somnifera, has been reported for its anti-inflammatory and anti-tumor effects. In this study, we show that Wit A enhances the ionizing radiation (IR)-induced apoptosis in human lymphoma U937 cells. Wit A-enhanced IR-induced apoptosis is associated with the PARP cleavage, caspase-3 activation, as well as specifically down-regulation of anti-apoptotic protein Bcl-2, suggesting that Wit A may be useful as a potential radiosensitizer. In addition, pretreatment of U937 cells with SP600125 (JNK inhibitor) or SB203589 (p38 MAPK inhibitor) dose-dependently inhibited the proteolytic cleavage of PARP. We provide the evidence here that generation of reactive oxygen species (ROS), Bcl-2 down-regulation and activation of MAPKs pathway are critically involved in the apoptosis induced by Wit A and radiation.-
dc.language.isoen-
dc.subject.MESHAntineoplastic Agents, Phytogenic/*pharmacology-
dc.subject.MESH*Apoptosis/drug effects/radiation effects-
dc.subject.MESHCombined Modality Therapy-
dc.subject.MESHDown-Regulation-
dc.subject.MESHHumans-
dc.subject.MESHJNK Mitogen-Activated Protein Kinases/metabolism-
dc.subject.MESHLymphoma, Large B-Cell, Diffuse/*drug therapy/metabolism/*radiotherapy-
dc.subject.MESHProto-Oncogene Proteins c-bcl-2/metabolism-
dc.subject.MESHReactive Oxygen Species/metabolism-
dc.subject.MESHU937 Cells-
dc.subject.MESHWithanolides/*pharmacology-
dc.subject.MESH*X-Ray Therapy-
dc.subject.MESHp38 Mitogen-Activated Protein Kinases/metabolism-
dc.titleCombination of withaferin A and X-ray irradiation enhances apoptosis in U937 cells.-
dc.typeArticle-
dc.identifier.pmid21964475-
dc.identifier.urlhttp://linkinghub.elsevier.com/retrieve/pii/S0887-2333(11)00258-X-
dc.contributor.affiliatedAuthor최, 경숙-
dc.type.localJournal Papers-
dc.identifier.doi10.1016/j.tiv.2011.09.016-
dc.citation.titleToxicology in vitro : an international journal published in association with BIBRA-
dc.citation.volume25-
dc.citation.number8-
dc.citation.date2011-
dc.citation.startPage1803-
dc.citation.endPage1810-
dc.identifier.bibliographicCitationToxicology in vitro : an international journal published in association with BIBRA, 25(8):1803-1810, 2011-
dc.identifier.eissn1879-3177-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Biochemistry & Molecular Biology
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