104 487

Cited 20 times in

Differential and cooperative actions of Olig1 and Olig2 transcription factors on immature proliferating cells after contusive spinal cord injury.

DC Field Value Language
dc.contributor.authorKim, HM-
dc.contributor.authorHwang, DH-
dc.contributor.authorChoi, JY-
dc.contributor.authorPark, CH-
dc.contributor.authorSuh-Kim, H-
dc.contributor.authorKim, SU-
dc.contributor.authorKim, BG-
dc.date.accessioned2012-04-24T06:24:24Z-
dc.date.available2012-04-24T06:24:24Z-
dc.date.issued2011-
dc.identifier.issn0894-1491-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/6542-
dc.description.abstractSpontaneous remyelination after spinal cord injury (SCI) is limited probably due to inadequate signaling to generate sufficient OLs from progenitor cells. The present study tested a hypothesis that introduction of olig genes, critical regulators of OL development, into immature proliferating cells could increase oligodendrogenesis after contusive SCI in adult rats. Recombinant retroviruses encoding Olig1 and Olig2 transcription factors, separately or in combination, with green fluorescent protein (GFP) were injected into the injured spinal cord. Unexpectedly, introduction of Olig2-GFP retroviruses led to a marked hyperplasia of GFP+ cells at 1 week, and soft agar colony forming assay of isolated GFP+ cells confirmed Olig2-induced tumorous transformation. In contrast, Olig1 did not alter the number of GFP+ cells. Simultaneous expression of Olig1 and Olig2 (Olig1/2) led to a marked increase in the number of GFP+ cells without tumor formation. The proportion of GFP+ cells with OL progenitor markers was increased by Olig1/2. Moreover, Olig1/2 robustly increased the proportion of mature OLs and expression of myelin related proteins, while Olig1 alone exhibited only modest effects. Olig1/2 upregulated Sox10, which drives terminal OL differentiation, implicating Sox 10 as a mediator of Olig1/2 effects on the maturation. Finally, injection of Olig1/2 retroviruses significantly improved a quality of hindpaws locomotion and increased the total number of OLs after SCI. Activation of both Olig1 and Olig2 may be beneficial by both increasing the progenitor cell proliferation and enhancing OL differentiation in the injured spinal cord.-
dc.language.isoen-
dc.subject.MESHAnalysis of Variance-
dc.subject.MESHAnimals-
dc.subject.MESHBasic Helix-Loop-Helix Transcription Factors/*metabolism-
dc.subject.MESHCell Count-
dc.subject.MESHCell Differentiation/*physiology-
dc.subject.MESH*Cell Proliferation-
dc.subject.MESHCells, Cultured-
dc.subject.MESHCerebral Cortex/cytology-
dc.subject.MESHColony-Forming Units Assay/methods-
dc.subject.MESHDisease Models, Animal-
dc.subject.MESHFemale-
dc.subject.MESHGreen Fluorescent Proteins/metabolism-
dc.subject.MESHLocomotion/physiology-
dc.subject.MESHNerve Tissue Proteins/*metabolism-
dc.subject.MESHNeuroglia/metabolism-
dc.subject.MESHRats-
dc.subject.MESHRats, Sprague-Dawley-
dc.subject.MESHRecovery of Function-
dc.subject.MESHSpinal Cord Injuries/metabolism/pathology/*physiopathology-
dc.subject.MESHStem Cells/physiology-
dc.subject.MESHTransfection/methods-
dc.titleDifferential and cooperative actions of Olig1 and Olig2 transcription factors on immature proliferating cells after contusive spinal cord injury.-
dc.typeArticle-
dc.identifier.pmid21538562-
dc.contributor.affiliatedAuthor황, 동훈-
dc.contributor.affiliatedAuthor최, 준영-
dc.contributor.affiliatedAuthor서, 해영-
dc.contributor.affiliatedAuthor김, 병곤-
dc.type.localJournal Papers-
dc.identifier.doi10.1002/glia.21182-
dc.citation.titleGlia-
dc.citation.volume59-
dc.citation.number7-
dc.citation.date2011-
dc.citation.startPage1094-
dc.citation.endPage1106-
dc.identifier.bibliographicCitationGlia, 59(7):1094-1106, 2011-
dc.identifier.eissn1098-1136-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Brain Science
Journal Papers > School of Medicine / Graduate School of Medicine > Neurology
Journal Papers > School of Medicine / Graduate School of Medicine > Anatomy
Files in This Item:
21538562.pdfDownload

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse