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Fully integrated lab-on-a-disc for simultaneous analysis of biochemistry and immunoassay from whole blood.

Authors
Lee, BS; Lee, YU; Kim, HS; Kim, TH; Park, J; Lee, JG; Kim, J; Kim, H; Lee, WG; Cho, YK
Citation
Lab on a chip, 11(1):70-78, 2011
Journal Title
Lab on a chip
ISSN
1473-01971473-0189
Abstract
We report a fully integrated device that can perform both multiple biochemical analysis and sandwich type immunoassay simultaneously on a disc. The whole blood is applied directly to the disposable "lab-on-a-disc" containing different kinds of freeze-dried reagents for the blood chemistry analysis as well as reagents required for the immunoassay. The concentrations of different kinds of analytes are reported within 22 min by simply inserting a disc to a portable device. Using the innovative laser irradiated ferrowax microvalves together with the centrifugal microfluidics, the total process of plasma separation, metering, mixing, incubation, washing, and detection is fully automated. The analyzer is equipped with an optical detection module to measure absorbances at 10 different wavelengths to accommodate the various kinds of reaction protocols. Compared to the conventional blood analysis done in clinical laboratories, it is advantageous for point-of-care applications because it requires a smaller amount of blood (350 μL vs. 3 mL), takes less time (22 min vs. several days), does not require specially trained operators or expensive instruments to run biochemical analysis and immunoassay separately.
MeSH terms
Blood Chemical Analysis/economics/*instrumentation/methodsEquipment DesignHumansImmunoassay/economics/*instrumentation/methodsMicrofluidic Analytical Techniques/economics/*instrumentation/methods
DOI
10.1039/c0lc00205d
PMID
21042620
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Laboratory Medicine
AJOU Authors
이, 위교
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