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Naegleria fowleri: nfa1 gene knock-down by double-stranded RNAs.

Authors
Jung, SY; Kim, JH; Lee, YJ; Song, KJ; Kim, K; Park, S; Im, KI; Shin, HJ
Citation
Experimental parasitology, 118(2):208-213, 2008
Journal Title
Experimental parasitology
ISSN
0014-48941090-2449
Abstract
Nfa1 protein expressed by the nfa1 gene that was cloned recently from pathogenic Naegleria fowleri was found in pseudopodia, especially food-cups, and concerned with a mechanism of pathogenicity of N. fowleri. In the present study, N. fowleri nfa1 gene was knocked down using double-stranded RNAs, and the expression of Nfa1 protein was observed. Using synthetic double-stranded RNA of the nfa1 gene in vitro, the nfa1 gene and Nfa1 protein were knocked down about 50.4+/-3.1% and 52+/-2%, respectively. These results suggest that RNA interference (RNAi) may be an effective technique for gene knock-down in N. fowleri trophozoites.
MeSH terms
AnimalsAntigens, Protozoan/genetics*Antigens, Protozoan/physiologyBlotting, NorthernBlotting, SouthernBlotting, WesternElectrophoresis, Agar GelGene Expression RegulationNaegleria fowleri/genetics*Protozoan Proteins/genetics*Protozoan Proteins/physiologyRNA Interference/physiology*RNA, Double-Stranded/genetics*Reverse Transcriptase Polymerase Chain ReactionTransfection
DOI
10.1016/j.exppara.2007.08.008
PMID
17904122
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
AJOU Authors
정, 석률송, 경주김, 경민박, 선신, 호준
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