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Regulation of multipotency and proliferation capability of human bone marrow-derived mesenchymal stem cells.

Lee, JE; Park, JS; Lee, YD; Suh-Kim, H; Kim, SS
Department of Anatomy
Human mesenchymal stem cell (hMSC) is an attractive source of adult stem cells for the therapeutic use in regenerative medicine. These cells are usually expanded in culture system prior to their application. However, during in vitro expansion, hMSC lose their multipotency and proliferation capability. The extent of multi-lineage differentiation ability of stem cells is believed to be associated with the expression of development- and differentiation- related genes. In this study, we investigated the effects of different culture conditions on the stem cell property of hMSCs. For this purpose, we first compared the proliferation rates and differentiation potential of hMSCs under different culture conditions (±bFGF). At first, expanded hMSCs by long-term culture show the enlargement morphology, proliferation arrest, and limited differentiation potential. Simultaneously, several development-related genes were found to be up- or down- regulated in hMSC depending on the progression of passage. Therefore, we specifically evaluated gene expression profile of Wnt pathway related genes. Interestingly, by overexpression or knockdown experiments, we found that some of genes were shown to be required for the proliferation of hMSCs. These findings suggest that development-related gene has a pivotal role in self-renewal of hMSCs during in vitro culture expansion.
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