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In vitro inhibition of coronavirus replications by the traditionally used medicinal herbal extracts, Cimicifuga rhizoma, Meliae cortex, Coptidis rhizoma, and Phellodendron cortex.

Authors
Kim, HY; Shin, HS; Park, H; Kim, YC; Yun, YG; Park, S; Shin, HJ; Kim, K
Citation
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology, 41(2):122-128, 2008
Journal Title
Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
ISSN
1386-65321873-5967
Abstract
BACKGROUND: A search for new anti-coronaviral drugs to treat coronaviral infections was motivated by an outbreak of severe acute respiratory syndrome (SARS). OBJECTIVES: In order to find drugs that treat coronavirus infections, including SARS, we screened traditional medicinal herbal extracts and evaluated their antiviral activities on coronavirus replication. STUDY DESIGN: We employed a plaque assay to evaluate the effect of 22 medicinal herbal extracts on virus replication. We determined the 50% effective concentration (EC50) of each extract that was necessary to inhibit the replication of mouse hepatitis virus A59 (MHV-A59); we also determined 50% cytotoxic concentrations (CC50) for each extract. Northern and Western blot analyzes were performed to investigate antiviral activity in MHV-infected DBT cells, including virus entry, viral RNA and protein expression, and virus release. Coronavirus specific inhibition was also demonstrated using porcine epidemic diarrhea virus (PEDV). RESULTS: Cimicifuga rhizoma, Meliae cortex, Coptidis rhizoma, Phellodendron cortex and Sophora subprostrata radix decreased the MHV production and the intracellular viral RNA and protein expression with EC50 values ranging from 2.0 to 27.5 microg/ml. These extracts also significantly decreased PEDV production and less dramatically decreased vesicular stomatitis virus (VSV) production in vitro. CONCLUSIONS: The extracts selected strongly inhibited MHV replication and could be potential candidates for new anti-coronavirus drugs.
MeSH terms
AnimalsAntiviral Agents/pharmacology*Cell LineCercopithecus aethiopsCimicifuga*HumansMurine hepatitis virus/drug effects*Murine hepatitis virus/physiologyPhellodendron*Plant Extracts/pharmacology*Plants, Medicinal*Vero CellsVirus Replication/drug effects*
DOI
10.1016/j.jcv.2007.10.011
PMID
18036887
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
AJOU Authors
박선신호준김경민
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