Methylation status of Histone H3 in the TIM-3 gene regulatory region
Komori, Kuniharu; Lee, kiyung; Park, Sun
Department of Microbiology
The T cell immunoglobulin domain and mucin domain-3 (TIM-3) was initially identified as a Th1-specific membrane protein. TIM-3 is critical one of the inhibitory receptor involved in regulating T cell responses, however, transcriptional regulation of TIM-3 has not been well studied. Given that transcriptional regulatory sequences are known to be conserved in different species, and to be dimethylated in histone H3 lysine 4 residue (H3K4), we examined H3 methylation status of TIM-3 upstream region in T cells. First, we compared sequences of TIM-3 upstream region in human, mouse and cow, and identified four conserved sequences, CNS 1, CNS 2, CNS 3 and CNS 4. H3K4 dimethylation was increased only in the CNS 4 by stimulation of T cells with phorbol 12-myristate 13-acetate and ionomycin that induces TIM-3 transcription. However, trimethylation of histone H3 lysine 9 residue was not altered in these four CNS. These results suggest possible relevance of CNS 4 to the TIM-3 transcription in T cells.
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