Cited 0 times in Scipus Cited Count

Early distribution of intravenously injected mesenchymal stem cells in rats with acute brain trauma evaluated by (99m)Tc-HMPAO labeling.

Authors
Park, BN  | Shim, W | Lee, G  | Bang, OY | An, YS  | Yoon, JK  | Ahn, YH
Citation
Nuclear medicine and biology, 38(8). : 1175-1182, 2011
Journal Title
Nuclear medicine and biology
ISSN
0969-80511872-9614
Abstract
INTRODUCTION: Stem cell tracking is essential for evaluation of its migration, transplantation and therapeutic response. The aim of this study was to evaluate early distribution of intravenously transplanted rat bone marrow mesenchymal stem cells (BMSCs) in rats with acute cerebral trauma by labeling with (99m)Tc-hexamethylpropyleneamine oxime ((99m)Tc-HMPAO).



METHODS: (99m)Tc-HMPAO-labeled BMSCs were injected intravenously to trauma rats (n=14) and sham-operated controls (n=13). Gamma camera images were acquired at 4 h after injection, and then organs were removed for gamma counting. Confocal microscope was used to confirm the migration of (99m)Tc-BMSCs by co-labeling with PKH26. Cytometric analysis was performed to evaluate apoptotic or necrotic change until the seventh day after labeling.



RESULTS: (99m)Tc-BMSCs were distributed mostly to lungs, liver and spleen at 4 h, and uptake of these organs was not significantly different between traumatic rats and controls. Meanwhile, the cerebral uptake of (99m)Tc-BMSCs was significantly higher in the traumatic rats than in controls (0.40% vs. 0.20%; P=.0002). Additionally, (99m)Tc-BMSCs' uptake of traumatic hemisphere was significantly higher than that of contralateral ones (0.27% vs. 0.13%; P=.0001) in traumatic rats. Regardless of radiolabeling, BMSCs migrated to traumatic regions, but not to nontraumatic hemispheres. However, gamma camera failed to demonstrate (99m)Tc-BMSCs in traumatic hemispheres. No significant apoptotic or necrotic change was observed until 7 days after radiolabeling.



CONCLUSIONS: Early distribution of BMSCs in traumatic brain disease could be monitored by (99m)Tc-labeling, which does not induce cellular death. However, our data showed that the amount of migrated (99m)Tc-BMSCs was not enough to be demonstrated by clinical gamma camera.
MeSH

DOI
10.1016/j.nucmedbio.2011.05.009
PMID
21831649
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Nuclear Medicine & Molecular Imaging
Journal Papers > School of Medicine / Graduate School of Medicine > Physiology
Journal Papers > School of Medicine / Graduate School of Medicine > Neurosurgery
Ajou Authors
박, 복남  |  안, 영실  |  안, 영환  |  윤, 준기  |  이, 광
Full Text Link
Files in This Item:
There are no files associated with this item.
Export

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse