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Heat shock protein 70 of Naegleria fowleri is important factor for proliferation and in vitro cytotoxicity.
DC Field | Value | Language |
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dc.contributor.author | Song, KJ | - |
dc.contributor.author | Song, KH | - |
dc.contributor.author | Kim, JH | - |
dc.contributor.author | Sohn, HJ | - |
dc.contributor.author | Lee, YJ | - |
dc.contributor.author | Park, CE | - |
dc.contributor.author | Shin, HJ | - |
dc.date.accessioned | 2010-12-17T05:45:48Z | - |
dc.date.available | 2010-12-17T05:45:48Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0932-0113 | - |
dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/673 | - |
dc.description.abstract | To evaluate the role of heat shock 70 protein (HSP70) in free-living amoeba, a constitutive and inducible heat shock 70 gene of pathogenic Naegleria fowleri has previously been cloned, characterized, and named as Nf-cHSP70. The Nf-cHSP70 is localized in the cytoplasm, pseudopodia, and phagocytic food-cups. To investigate the role of Nf-cHSP70 in the pathogenicity of N. fowleri, the synthesis of N. fowleri HSP70 was first inhibited with benzylidene lactam compound (KNK437), and Nf-cHSP70 gene was knock-downed with antisense oligomers, which were designed with a start region-specific antisense oligonucleotides (24 oligomers) and modified with phosphorothioate. KNK437 inhibited the induction of N. fowleri HSP70 in a dose-dependent manner. In addition, 300 muM KNK437 reduced the proliferation of N. fowleri to 79.4% of untreated control (100%). Nf-cHSP70 knock-downed N. fowleri with antisense oligomers showed 68.5% reduction of proliferation in comparison with untreated control (100%). The cytotoxicity of N. fowleri against CHO target cells was reduced to 42.1% by KNK437 and 68.6% by antisense oligomers. These results suggest that the cloned Nf-cHSP70 plays an important role in the proliferation and cytotoxicity of pathogenic N. fowleri. | - |
dc.language.iso | en | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Benzhydryl Compounds | - |
dc.subject.MESH | CHO Cells | - |
dc.subject.MESH | Cricetinae | - |
dc.subject.MESH | Cricetulus | - |
dc.subject.MESH | Gene Deletion | - |
dc.subject.MESH | HSP70 Heat-Shock Proteins | - |
dc.subject.MESH | Naegleria fowleri | - |
dc.subject.MESH | Protozoan Proteins | - |
dc.subject.MESH | Pyrrolidinones | - |
dc.subject.MESH | Trophozoites | - |
dc.title | Heat shock protein 70 of Naegleria fowleri is important factor for proliferation and in vitro cytotoxicity. | - |
dc.type | Article | - |
dc.identifier.pmid | 18421477 | - |
dc.contributor.affiliatedAuthor | 신, 호준 | - |
dc.type.local | Journal Papers | - |
dc.identifier.doi | 10.1007/s00436-008-0972-x | - |
dc.citation.title | Parasitology research | - |
dc.citation.volume | 103 | - |
dc.citation.number | 2 | - |
dc.citation.date | 2008 | - |
dc.citation.startPage | 313 | - |
dc.citation.endPage | 317 | - |
dc.identifier.bibliographicCitation | Parasitology research, 103(2). : 313-317, 2008 | - |
dc.identifier.eissn | 1432-1955 | - |
dc.relation.journalid | J009320113 | - |
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