21 243

Cited 10 times in

Modulation of hepatitis B virus replication by expression of polymerase-surface fusion protein through splicing: implications for viral persistence.

Authors
Park, GS; Kim, HY; Shin, HS; Park, S; Shin, HJ; Kim, K
Citation
Virus research, 136(1-2):166-174, 2008
Journal Title
Virus research
ISSN
0168-17021872-7492
Abstract
In hepatitis B virus (HBV)-infected livers and -transfected hepatoma cells, spliced transcripts are not essential for HBV replication. However, their ability to modulate HBV replication has not been clearly elucidated. In the current study, we found that the polymerase-surface (PS) fusion protein, generated from a spliced HBV transcript, colocalized with the nuclear pore complex, vimentin, microtubules, and the endoplasmic reticulum (ER) in the perinuclear region of transfected cells. We found that PLC/PRF/5-hepatoma cells expressed PS transcript and PS protein. Hepatitis B surface antigen (HBs) secretion, core particle formation, and HBV DNA synthesis were inhibited by the expression of PS transcript and PS protein, and by expression of PS transcript alone, suggesting that HBV replication is modulated by splicing. Our results suggest that splicing may be one of the outcomes of the host-virus interaction.
MeSH terms
Cell LineCytoplasm/chemistryEndoplasmic Reticulum/chemistryGene Products, pol/biosynthesis*Hepatitis B Surface Antigens/biosynthesis*Hepatitis B virus/physiology*Hepatocytes/chemistryHepatocytes/virologyHumansMicrotubules/chemistryRNA Splicing*Vimentin/metabolismViral Fusion Proteins/biosynthesis*Virus AssemblyVirus Replication*
DOI
10.1016/j.virusres.2008.05.005
PMID
18562032
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
AJOU Authors
박선신호준김경민
Full Text Link
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse