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Green tea prevents down-regulation of gap junction intercellular communication in human keratinocytes treated with PMA.

Authors
Choung, YH; Choi, SJ; Joo, JS; Lee, JB; Lee, HK; Lee, SJ
Citation
European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery, 268(6):885-892, 2011
Journal Title
European archives of oto-rhino-laryngology : official journal of the European Federation of Oto-Rhino-Laryngological Societies (EUFOS) : affiliated with the German Society for Oto-Rhino-Laryngology - Head and Neck Surgery
ISSN
0937-44771434-4726
Abstract
It has been suggested that connexin (Cx) gap junction proteins act as tumor suppressors and green tea has a potential to prevent tumor development, however, the studies on their association with human keratinocytes were rare. We evaluated the effects of a tumor promoter, phorbol-12-myristate-13-acetate (PMA), on the expression of Cxs and gap junction intercellular communication (GJIC) in human keratinocytes (HaCaT cells) and explored the preventive effects of green tea extracts-epicatechin (EC) and epigallocatechin-3-gallate (EGCG). We performed neutral red dye uptake assay to determine the optimal concentrations of PMA, EC, and EGCG for this study and confirmed the expression of Cx mRNAs using RT-PCR. We evaluated GJIC quantitatively using the 'scrape-loading dye transfer (SLDT)' technique after 24-h culture of HaCaT cells treated with agents. To analyze the expression change of Cxs, we also performed Western blot and immunocytochemistry. HaCaT cells were found to express Cx26, Cx30, Cx31, and Cx43, but not Cx29. In 'scrape-loading dye transfer' for functional study for GJIC, EC and EGCG significantly prevented PMA-induced down-regulation of GJIC. Western blot analyses revealed that EC and EGCG prevented down-regulation of Cx26 and Cx43 proteins in HaCaT cells treated with PMA. Immunocytochemistry showed decreased expression and abnormal location of Cx26 and Cx43 in HaCaT cells when treated with PMA, and EC and EGCG inhibited its effect. These results suggest an important role of GJIC played in carcinogenesis involving human keratinocytes and green tea as a useful anticancer diet.
MeSH terms
Blotting, WesternCell Communication/*drug effects/geneticsConnexins/biosynthesis/geneticsDown-Regulation/*geneticsGap Junctions/*drug effects/metabolismHumansImmunohistochemistryKeratinocytes/drug effects/metabolism/*pathologyLaryngeal Neoplasms/pathology/prevention & controlPhorbol Esters/*pharmacologyPlant Extracts/*pharmacologyRNA, Messenger/*geneticsReverse Transcriptase Polymerase Chain ReactionTeaTumor Cells, Cultured
DOI
10.1007/s00405-010-1411-z
PMID
21042803
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Otolaryngology
AJOU Authors
정, 연훈
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