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Green tea (-)-epigallocatechin-3-gallate inhibits HGF-induced progression in oral cavity cancer through suppression of HGF/c-Met.

Authors
Koh, YW; Choi, EC; Kang, SU; Hwang, HS; Lee, MH; Pyun, J; Park, R; Lee, Y; Kim, CH
Citation
The Journal of nutritional biochemistry, 22(11):1074-1083, 2011
Journal Title
The Journal of nutritional biochemistry
ISSN
0955-28631873-4847
Abstract
Hepatocyte growth factor (HGF) and c-Met have recently attracted a great deal of attention as prognostic indicators of patient outcome, and they are important in the control of tumor growth and invasion. Epigallocatechin-3-gallate (EGCG) has been shown to modulate multiple signal pathways in a manner that controls the unwanted proliferation and invasion of cells, thereby imparting cancer chemopreventive and therapeutic effects. In this study, we investigated the effects of EGCG in inhibiting HGF-induced tumor growth and invasion of oral cancer in vitro and in vivo. We examined the effects of EGCG on HGF-induced cell proliferation, migration, invasion, induction of apoptosis and modulation of HGF/c-Met signaling pathway in the KB oral cancer cell line. We investigated the antitumor effect and inhibition of c-Met expression by EGCG in a syngeneic mouse model (C3H/HeJ mice, SCC VII/SF cell line). HGF promoted cell proliferation, migration, invasion and induction of MMP (matrix metalloproteinase)-2 and MMP-9 in KB cells. EGCG significantly inhibited HGF-induced phosphorylation of Met and cell growth, invasion and expression of MMP-2 and MMP-9. EGCG blocked HGF-induced phosphorylation of c-Met and that of the downstream kinases AKT and ERK, and inhibition of p-AKT and p-ERK by EGCG was associated with marked increases in the phosphorylation of p38, JNK, cleaved caspase-3 and poly-ADP-ribose polymerase. In C3H/HeJ syngeneic mice, as an in vivo model, tumor growth was suppressed and apoptosis was increased by EGCG. Our results suggest that EGCG may be a potential therapeutic agent to inhibit HGF-induced tumor growth and invasion in oral cancer.
MeSH terms
AnimalsCatechin/*analogs & derivatives/pharmacologyCell Movement/drug effectsCell Proliferation/drug effectsChemopreventionDisease ProgressionFemaleHepatocyte Growth Factor/*antagonists & inhibitorsHumansKB CellsMatrix Metalloproteinase 2/biosynthesisMatrix Metalloproteinase 9/biosynthesisMiceMouth Neoplasms/pathologyNeoplasm InvasivenessProto-Oncogene Proteins c-met/*antagonists & inhibitors/biosynthesisSignal Transduction/drug effectsTea
DOI
10.1016/j.jnutbio.2010.09.005
PMID
21292466
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Otolaryngology
Journal Papers > School of Medicine / Graduate School of Medicine > Anatomy
AJOU Authors
이, 영돈김, 철호
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