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Identification of a peptide that interacts with Nestin protein expressed in brain cancer stem cells.

Authors
Beck, S; Jin, X; Yin, J; Kim, SH; Lee, NK; Oh, SY; Kim, MK; Kim, EB; Son, JS; Kim, SC; Nam, DH; Kang, SK; Kim, H; Choi, YJ
Citation
Biomaterials, 32(33):8518-8528, 2011
Journal Title
Biomaterials
ISSN
0142-96121878-5905
Abstract
Glioma stem cells (GSCs) are presumably major culprits for brain tumor initiation, progression, and recurrence after conventional therapies. Thus, selective targeting and eradication of GSCs may provide a promising and effective therapeutic approach. Here, we isolated a GSC-targeting (GSCT) peptide that demonstrated selective binding affinity for many undifferentiated GSCs using in vitro phage display technology. This GSCT peptide binds to isotypes of Nestin proteins specifically expressed in GSCs, enabling it to target Nestin-positive cells in human glioblastoma tissues. In human glioblastoma tissue specimens, the fluorescence-conjugated GSCT peptide could visualize putative GSC populations, showing its possible use as a diagnostic agent. GSCT peptide is also internalized into undifferentiated GSCs specifically in vitro, and moreover, intravenously injected GSCT peptide effectively penetrated into tissues, specifically accumulated in gliomas that arise from subcutaneous and orthotopic implantation, and predominantly targeted Nestin-positive cells in these tumors. Thus, our GSCT peptide may be useful for the development of more promising therapeutic and diagnostic modalities that target GSCs in brain tumors.
MeSH terms
AnimalsBrain Neoplasms/*metabolism/pathologyCell DifferentiationCell Line, TumorFluorescent Antibody TechniqueGlioblastoma/*metabolism/pathologyHumansIntermediate Filament Proteins/*metabolismMiceMice, Inbred BALB CNeoplastic Stem Cells/*metabolismNerve Tissue Proteins/*metabolismPeptides/*metabolismProtein Binding
DOI
10.1016/j.biomaterials.2011.07.048
PMID
21880363
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Neurosurgery
AJOU Authors
김, 세혁
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