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Expression of the capsid protein of Chikungunya virus in a baculovirus for serodiagnosis of Chikungunya disease.

Authors
Cho, B; Kim, J; Cho, JE; Jeon, BY; Park, S
Citation
Journal of virological methods, 154(1-2):154-159, 2008
Journal Title
Journal of virological methods
ISSN
0166-09341879-0984
Abstract
Chikungunya virus (CHIKV) causes endemic or epidemic outbreaks of CHIK fever, which typically manifests as a febrile illness. To develop a CHIKV-specific diagnostic test, CHIKV capsid protein was expressed using a baculovirus expression system. The seroreactvity of the recombinant CHIKV capsid protein was evaluated by ELISA and immuochromatographic assay (ICA), using 40 anti-CHIKV-positive and 20 anti-CHIKV-negative sera, an additional 20 normal sera samples from healthy Koreans, and 20 anti-Dengue virus sera samples. The sensitivity of the recombinant CHIKV capsid protein was 85% and 87.5% as measured by ELISA and ICA, respectively. The specificity of the recombinant CHIKV capsid protein was 100% both by ELISA and by ICA. No cross-reactivity of the capsid protein was seen with anti-Dengue virus sera samples. There was a significant correlation between the ELISA- and ICA-measured seroreactivities of the recombinant CHIKV capsid protein for anti-CHIKV IgM-positive sera samples. These results suggest that the recombinant CHIKV capsid protein could be used in a diagnostic test for identifying CHIKV disease.
MeSH terms
Alphavirus Infections/diagnosis*Antibodies, Viral/blood*Antigens, Viral/diagnostic use*Antigens, Viral/geneticsAntigens, Viral/isolation & purificationBaculoviridae/geneticsCapsid Proteins/diagnostic use*Capsid Proteins/geneticsCapsid Proteins/isolation & purificationChikungunya virus/geneticsChikungunya virus/immunology*Cross ReactionsGene ExpressionHumansImmunoassay/methodsImmunoglobulin M/bloodKoreaRecombinant Proteins/diagnostic useRecombinant Proteins/geneticsRecombinant Proteins/isolation & purificationSensitivity and Specificity
DOI
10.1016/j.jviromet.2008.07.031
PMID
18761376
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Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
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