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Expression of the capsid protein of Chikungunya virus in a baculovirus for serodiagnosis of Chikungunya disease.
DC Field | Value | Language |
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dc.contributor.author | Cho, B | - |
dc.contributor.author | Kim, J | - |
dc.contributor.author | Cho, JE | - |
dc.contributor.author | Jeon, BY | - |
dc.contributor.author | Park, S | - |
dc.date.accessioned | 2010-12-17T06:48:19Z | - |
dc.date.available | 2010-12-17T06:48:19Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0166-0934 | - |
dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/683 | - |
dc.description.abstract | Chikungunya virus (CHIKV) causes endemic or epidemic outbreaks of CHIK fever, which typically manifests as a febrile illness. To develop a CHIKV-specific diagnostic test, CHIKV capsid protein was expressed using a baculovirus expression system. The seroreactvity of the recombinant CHIKV capsid protein was evaluated by ELISA and immuochromatographic assay (ICA), using 40 anti-CHIKV-positive and 20 anti-CHIKV-negative sera, an additional 20 normal sera samples from healthy Koreans, and 20 anti-Dengue virus sera samples. The sensitivity of the recombinant CHIKV capsid protein was 85% and 87.5% as measured by ELISA and ICA, respectively. The specificity of the recombinant CHIKV capsid protein was 100% both by ELISA and by ICA. No cross-reactivity of the capsid protein was seen with anti-Dengue virus sera samples. There was a significant correlation between the ELISA- and ICA-measured seroreactivities of the recombinant CHIKV capsid protein for anti-CHIKV IgM-positive sera samples. These results suggest that the recombinant CHIKV capsid protein could be used in a diagnostic test for identifying CHIKV disease. | - |
dc.language.iso | en | - |
dc.subject.MESH | Alphavirus Infections | - |
dc.subject.MESH | Antibodies, Viral | - |
dc.subject.MESH | Antigens, Viral | - |
dc.subject.MESH | Baculoviridae | - |
dc.subject.MESH | Capsid Proteins | - |
dc.subject.MESH | Chikungunya virus | - |
dc.subject.MESH | Cross Reactions | - |
dc.subject.MESH | Gene Expression | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Immunoassay | - |
dc.subject.MESH | Immunoglobulin M | - |
dc.subject.MESH | Korea | - |
dc.subject.MESH | Recombinant Proteins | - |
dc.subject.MESH | Sensitivity and Specificity | - |
dc.title | Expression of the capsid protein of Chikungunya virus in a baculovirus for serodiagnosis of Chikungunya disease. | - |
dc.type | Article | - |
dc.identifier.pmid | 18761376 | - |
dc.identifier.url | http://linkinghub.elsevier.com/retrieve/pii/S0166-0934(08)00293-0 | - |
dc.contributor.affiliatedAuthor | 박, 선 | - |
dc.type.local | Journal Papers | - |
dc.identifier.doi | 10.1016/j.jviromet.2008.07.031 | - |
dc.citation.title | Journal of virological methods | - |
dc.citation.volume | 154 | - |
dc.citation.number | 1-2 | - |
dc.citation.date | 2008 | - |
dc.citation.startPage | 154 | - |
dc.citation.endPage | 159 | - |
dc.identifier.bibliographicCitation | Journal of virological methods, 154(1-2). : 154-159, 2008 | - |
dc.identifier.eissn | 1879-0984 | - |
dc.relation.journalid | J001660934 | - |
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