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Contact-independent cell death of human microglial cells due to pathogenic Naegleria fowleri trophozoites.
DC Field | Value | Language |
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dc.contributor.author | Kim, JH | - |
dc.contributor.author | Kim, D | - |
dc.contributor.author | Shin, HJ | - |
dc.date.accessioned | 2010-12-17T06:53:45Z | - |
dc.date.available | 2010-12-17T06:53:45Z | - |
dc.date.issued | 2008 | - |
dc.identifier.issn | 0023-4001 | - |
dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/684 | - |
dc.description.abstract | Free-living Naegleria fowleri leads to a fatal infection known as primary amebic meningoencephalitis in humans. Previously, the target cell death could be induced by phagocytic activity of N. fowleri as a contact-dependent mechanism. However, in this study we investigated the target cell death under a non-contact system using a tissue-culture insert. The human microglial cells, U87MG cells, co-cultured with N. fowleri trophozoites for 30 min in a non-contact system showed morphological changes such as the cell membrane destruction and a reduction in the number. By fluorescence-activated cell sorter (FACS) analysis, U87MG cells co-cultured with N. fowleri trophozoites in a non-contact system showed a significant increase of apoptotic cells (16%) in comparison with that of the control or N. fowleri lysate. When U87MG cells were co-cultured with N. fowleri trophozoites in a non-contact system for 30 min, 2 hr, and 4 hr, the cytotoxicity of amebae against target cells was 40.5, 44.2, and 45.6%, respectively. By contrast, the cytotoxicity of non-pathogenic N. gruberi trophozoites was 10.2, 12.4, and 13.2%, respectively. These results suggest that the molecules released from N. fowleri in a contact-independent manner as well as phagocytosis in a contact-dependent manner may induce the host cell death. | - |
dc.language.iso | en | - |
dc.subject.MESH | Animals | - |
dc.subject.MESH | Apoptosis | - |
dc.subject.MESH | Cell Line | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Microglia | - |
dc.subject.MESH | Naegleria fowleri | - |
dc.subject.MESH | Phagocytosis | - |
dc.title | Contact-independent cell death of human microglial cells due to pathogenic Naegleria fowleri trophozoites. | - |
dc.type | Article | - |
dc.identifier.pmid | 19127326 | - |
dc.identifier.url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2612605/ | - |
dc.contributor.affiliatedAuthor | 신, 호준 | - |
dc.type.local | Journal Papers | - |
dc.identifier.doi | 10.3347/kjp.2008.46.4.217 | - |
dc.citation.title | The Korean journal of parasitology | - |
dc.citation.volume | 46 | - |
dc.citation.number | 4 | - |
dc.citation.date | 2008 | - |
dc.citation.startPage | 217 | - |
dc.citation.endPage | 221 | - |
dc.identifier.bibliographicCitation | The Korean journal of parasitology, 46(4). : 217-221, 2008 | - |
dc.identifier.eissn | 1738-0006 | - |
dc.relation.journalid | J000234001 | - |
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