60 252

Cited 12 times in

Potential induction of rat muscle-derived stem cells to neural-like cells by retinoic acid.

Authors
Kim, ES; Kim, GH; Kang, ML; Kang, YM; Kang, KN; Hwang, KC; Min, BH; Kim, JH; Kim, MS
Citation
Journal of tissue engineering and regenerative medicine, 5(5):410-414, 2011
Journal Title
Journal of tissue engineering and regenerative medicine
ISSN
1932-62541932-7005
Abstract
Several recent studies have demonstrated that stem cell differentiation can be generated by derivatives of retinoic acid. In this study we chose retinoic acid (RA) for inducing neural differentiation of rat muscle-derived stem cells (rMDSCs). rMDSCs were pre-induced with 10 ng/ml basic fibroblast growth factor (bFGF) and then treated with 2 μM RA. After stimulation, RA induced rMDSCs to have a neural-like morphology after 1-7 days of in vitro differentiation. In the results of immunocytochemistry, rMDSC treated with RA showed abundant positive cells against the neuronal markers neuronal-specific enolase (NSE) and tubulin-βIII (Tuj1). Also, 2',3'-cyclic nucleotide 3'-phosphodiesterase (CNPase)-positive cells were observed, indicating oligodendrocyte lineage cells. However, positive cells against glial fibrillary acidic protein (GFAP), marker of astrocytes, were not detected. The mRNA profile of these cells included higher expression of NSE compared with those of non-treated cells in real-time PCR. From the data in this work, we suggest that rMDSCs can trans-differentiate into a neural-like phenotype under the RA conditions.
MeSH terms
AnimalsAstrocytes/cytologyCell DifferentiationCell LineageFemaleGene Expression ProfilingGlial Fibrillary Acidic Protein/metabolismImmunohistochemistry/methodsMuscles/cytologyNeurogenesisNeurons/metabolismOligodendroglia/cytologyRatsRats, Inbred F344Reverse Transcriptase Polymerase Chain ReactionStem Cells/*cytologyTretinoin/*metabolism
DOI
10.1002/term.328
PMID
20740690
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Orthopedic Surgery
AJOU Authors
민, 병현
Files in This Item:
fulltext not available.txtDownload
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse