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Implantation of bone marrow-derived buffy coat can supplement bone marrow stimulation for articular cartilage repair.

Authors
Jin, LH; Choi, BH; Kim, YJ; Park, SR; Jin, CZ; Min, BH
Citation
Osteoarthritis and cartilage, 19(12):1440-1448, 2011
Journal Title
Osteoarthritis and cartilage
ISSN
1063-45841522-9653
Abstract
OBJECTIVE: Bone marrow stimulation (BMS) has been regarded as a first line procedure for repair of articular cartilage. However, repaired cartilage from BMS is known to be unlike that of hyaline cartilage and its inner endurance is not guaranteed. The reason presumably came from a shortage of cartilage-forming cells in blood clots derived by BMS. In order to increase repairable cellularity, the feasibility of autologous bone marrow-derived buffy coat transplantation in repair of large full-thickness cartilage defects was investigated in this study.



METHODS: Rabbits were divided into four groups: the defect remained untreated as a negative control; performance of BMS only (BMS group); BMS followed by supplementation of autologous bone marrow buffy coat (Buffy coat group); transplantation of autologous osteochondral transplantation (AOTS) as a positive control.



RESULTS: Repair of cartilage defects in the Buffy coat group in a rabbit model was more effective than BMS alone and similar to AOTS. Gross findings, histological analysis, histological scoring, immunohistochemistry, and chemical assay demonstrated that supplementation of autologous bone marrow buffy coat after BMS arthroplasty effectively repaired cartilage defects in a rabbit model, and was more effective than BMS arthroplasty alone.



CONCLUSION: Supplementation of autologous bone marrow-derived buffy coat in cases of BMS could be a useful clinical protocol for cartilage repair.
MeSH terms
AnimalsBlood Buffy Coat/*transplantationBone Marrow Cells/*physiologyCartilage, Articular/*injuries/metabolism/pathologyCentrifugation, Density GradientCollagen Type II/metabolismColony-Forming Units AssayDisease Models, AnimalExtracellular Matrix/transplantationFeasibility StudiesGlycosaminoglycans/metabolismMesenchymal Stromal Cells/physiologyRabbits
DOI
10.1016/j.joca.2011.07.012
PMID
21843651
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Orthopedic Surgery
Journal Papers > Research Organization > Cell Therapy Center
AJOU Authors
김, 영직민, 병현
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