6 237

Cited 15 times in

Altered expression and localization of connexin32 in human and murine gastric carcinogenesis.

Authors
Jee, H; Nam, KT; Kwon, HJ; Han, SU; Kim, DY
Citation
Digestive diseases and sciences, 56(5):1323-1332, 2011
Journal Title
Digestive diseases and sciences
ISSN
0163-21161573-2568
Abstract
BACKGROUND: Intercellular communication via gap junctions, composed of protein subunits called connexins (Cxs), plays a key role in controlling cell growth, differentiation and carcinogenesis. Impaired gap junctional intercellular communication has been reported in various cancers and diseases.



AIMS: We investigated Cx32 expression patterns and semiquantitatively assessed Cx32 expression in cancers and preneoplastic lesions. To determine if cell proliferation is correlated with Cx32 expression, we evaluated Ki67 expression in a gastric cancer mouse model.



METHODS: In human and mouse, normal stomach and gastric adenocarcinoma tissues were used for immunohistochemical analyses.



RESULTS: Cx32 was detected at cell-cell (intercellular) contact points in normal cells and exhibited punctate intercellular and intracytoplasmic staining in cancer cells. The frequency of Cx32 loss of expression was significantly higher in human adenocarcinomas than in normal stomach. As tumor cells were less differentiated, Cx32 expression levels and intercellular and intracytoplasmic staining were also significantly lower. The Cx32 expression pattern in the mouse gastric cancer model was similar in several important respects to that of human. In mucous metaplasia of the mouse stomach, Cx32 was mainly expressed in the cytoplasm of epithelial cells. There was also an inverse correlation between Cx32 expression and cell proliferation in mouse tumors. However, there was no difference in the levels of Cx32 mRNA between normal and cancerous tissues.



CONCLUSIONS: These findings suggest that altered Cx32 expression, a loss of intercellular Cx32 and a gain of intracytoplasmic Cx32 in the form of punctate "dot", plays an important role in the formation of gastric adenocarcinomas.
MeSH terms
AnimalsConnexins/genetics/*metabolismGene Expression Regulation, Neoplastic/*physiologyHumansKi-67 Antigen/genetics/metabolismMaleMiceMice, Inbred C57BLRNA, Messenger/genetics/metabolismStomach Neoplasms/*metabolism
DOI
10.1007/s10620-010-1467-z
PMID
21082351
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Surgery
AJOU Authors
한, 상욱
Export
RIS (EndNote)
XLS (Excel)
XML

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse