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Production of monoclonal antibodies for rapid diagnostic kit of Influenza viruses

Sim, Moon-Hee; Park, Soo-Jeong; Lee, Jinyoung; Sohn, Hae-Jin; Kim, Jong-Hyun; Shin, Ho-Joon
Department of Microbiology
Influenza is an infectious disease which caused by RNA virus of the family Orthomyxoviridae that can affect birds, pigs and humans. Influenza viruses spread across the entire world in seasonal epidemics, leading to death between 250,000 and 500,000 people every year and up to millions people in some pandemic years. These numerous deaths indicate that definitive diagnosis was significantly difficult and may aggravate the disease during the epidemic waves. Effective ways to reduce the influenza pandemics include rapid diagnosis, quickly segregation from non-influenza cases as well as personal hygiene habits. However, there are many technology limitations up to now in order to early diagnose the disease. RT-PCR is considered an ideal methodology due to very high sensitivity but it should be used under selected circumstances. Currently, it has been used Rapid Influenza Diagnostic Test (RIDT) that is very easy and swift to perform, but it shows very low sensitivity and specificity. Monoclonal antibodies (mAb) have been used in variety of diagnostic methods. Therefore, it is necessary for producing of mAb with high sensitivity and specificity to diagnose influenza viruses. It may be very useful for the initial screening to distinguish pandemic and seasonal influenza viruses. We produced hybridomas secreting mAbs against H1N1 seasonal and H1N1 pandemic type. BALB/c mice were immunized with antigens (A/Brisbane/57/2007 like virus and A/California/7/2009 like virus) and then, the mAbs were characterized by isotyping analysis. The isotypes of mAbs were IgG2b and Kappa light chain. Although we need the investigation of mAbs against specimens of patients infected with influenza, these results suggest that our mAbs may be useful for developing high sensitive and specific RIDT kit.
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