Decreased Lactate Dehydrogenase B Expression Triggers Mitochondrial Respiratory Defects and Delayed Cell Growth
Lee, Young-Kyoung; Kim, June-Hyung; Yoon, Gyesoon
Department of Biochemistry & Molecular Biology
Aerobic glycolysis (i.e. the Warburg effect) is a distinctive hallmark of solid tumors. We recently reported that increased glycolytic lactate production of hepatoma cells was linked with decreased lactate dehydrogenase (LDH) B expression and mitochondrial respiratory defects. In this study, we aimed to elucidate whether and how LDHB suppression contributes to mitochondrial dysfunction.
When we suppressed LDHB expression by siRNA-mediated knockdown, we clearly observed
decreased cellular oxygen consumption rate and delayed cell growth. Unexpectedly, we observed that in the hepatoma cells with low level of LDHB, such as SNU354 and SNU423, LDHA was not only located in cytoplasm but also in nucleus and mitochondria. LDHA translocation into mitochondria and nucleus was also observed by siRNA-mediated LDHB knockdown, implying that LDHA translocation may be associated with mitochondrial dysfunction. Taken together, our results
suggest that aerobic glycolysis and mitochondrial dysfunction, key metabolic signatures of cancer cell, can be triggered or maintained by suppressing LDHB during tumorigenesis.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
Total Visit :2,795,042
Total Download :1,138,384
Today View :1,105
Ajou University Medical Information & Media Center 164 Worldcup-ro Yeongtong-gu Suwon 16499 Korea / TEL : 031-219-5312 / FAX : 031-219-5314 Copyright (c) Ajou University Medical Information & Media Center All Rights Reserved. AJOU Open Repository는 국립중앙도서관 OAK 보급사업으로 구축되었습니다.