Mitochondrial respiratory defects is linked with hepatoma cell invasiveness through claudin-1 induction
Lee, Jong-Hyuk; Kim, June-Hyung; Yoon, Gyesoon
Department of Biochemistry & Molecular Biology, Ajou University School of Medicine
Claudins are tight junction proteins that have recently been reported to be involved in cancer metastasis. Interestingly, we found that SNU human hepatoma cells with mitochondrial dysfunction have high level of Claudin-1(Cln-1) expression. In those HCC cells, Cln-1 played a key role in regulating invasiveness. Claudin-1 induction was also observed in human hepatocellular carcinoma tissues. Thus, we screened whether any specific mitochondrial defect was linked with Cln-1 induction. Among diverse respiratory inhibitors, complex I inhibition by rotenone most effectively induced Cln-1 expression at transcriptional level. This complex I defect-mediated Cln-1 induction was further confirmed by employing knockdown of NDUFA9, one of complex I subunits. These results suggest that mitochondrial respiratory defects are involved in cancer cell invasiveness through Cln-1 induction.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
Total Visit :5,272,742
Total Download :2,223,359
Today View :1,652
Ajou University Medical Information & Media Center 164 Worldcup-ro Yeongtong-gu Suwon 16499 Korea / TEL : 031-219-5312 / FAX : 031-219-5314 Copyright (c) Ajou University Medical Information & Media Center All Rights Reserved. AJOU Open Repository는 국립중앙도서관 OAK 보급사업으로 구축되었습니다.