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Increased proteolytic activity and ROS generation from Hop J pollens with environmental changes

Lee, Seung Ihm; Shin, Yoo Sub; Kim, Seung Hyun; Jin, Hyun Jung; Park, Hae Sim
Department of Allergy & Clinical Immunology
Background: Pollinosis is increasing with environmental changes in this country. We reported that the allergenicity of Hop J pollen has been increasing in recent 10 years. Objective: To evaluate the changes of proteolytic activity and ROS generation by Hop J pollen with environmental changes. Methods: We prepared two different Hop J pollen extracts collected in Suwon in 1998 and 2009(98, 09 extracts). Protease activity was analyzed using gelatin zymography. Bronchial epithelial cells(Calu-3) were cultured using air interfaced culture system to analyze transepithelial permeability assay and, ROS generation using 2’,7’-dichlorofluorescin diacetate (DCFDA).

Results: Both 98 and 09 extracts showed strong proteolytic activity, while transepithelial permeability through disruption of cell barrier protein increased significantly in 09 extracts compared to 98 extracts. The ROS generation was significantly higher in 09 extracts than in 98 extracts. Conclusion: Environmental changes could increase proteolytic activity and ROS generation of Hop J pollen, augmenting transepithelial permeability and airway inflammation.
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