A quantitative lateral flow immunoassay for the determination of cartilage oligomeric matrix protein as a marker of osteoarthritis
Hong, Sung Yub; Park, Yoo Min; Jang, Yo Han; Min, Byoung-Hyun; Yoon, Hyun C.
Department of Orthopedic Surgery, Ajou University School of Medicine
This study reports a simple and quantitative analytical method for cartilage oligomeric matrix protein (COMP) using a lateral flow immunoassay. An immunochromatographic assay using gold nanoparticle (AuNPs)-labeled monoclonal antibody (12C4) probe for the quantitative detection of COMP in human synovial fluid was developed. The detection antibody was conjugated with AuNPs (30 nm) as a detector, and the capture antibody (16F12) was immobilized within a multi-spot pattern on the test zone of the membrane strip. The COMP in human synovial fluid binds with AuNP-conjugated detection antibody and then COMP-Ab complex binds to the capture antibody. The immunoassay test can be analyzed by visualizing the test strip with a commercial scanner and an imaging program. The color density of the multi-spot pattern at the test zone was increased in proportion to the concentration of COMP. The obtained results from the developed immunoassay exhibited a dynamic COMP detection range between 0.6 μg/mL and 10 μg/mL. The detection limit of the proposed lateral flow immunoassay system was about 0.2 μg/mL. Results from the developed analysis system were compared with a conventional ELISA. Signal from the developed analysis system and ELISA showed a good correlation. The assay time of the quantitative test of COMP was less than 5 min. We expect that the proposed immunoassay method is suitable for the practical osteoarthritis diagnosis based on the quantification of COMP.
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.
Total Visit :3,824,509
Total Download :1,608,511
Today View :100
Ajou University Medical Information & Media Center 164 Worldcup-ro Yeongtong-gu Suwon 16499 Korea / TEL : 031-219-5312 / FAX : 031-219-5314 Copyright (c) Ajou University Medical Information & Media Center All Rights Reserved. AJOU Open Repository는 국립중앙도서관 OAK 보급사업으로 구축되었습니다.