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Combined effect of IL-10 and TGF-beta1 promoter polymorphisms as a risk factor for aspirin-intolerant asthma and rhinosinusitis.
DC Field | Value | Language |
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dc.contributor.author | Kim, SH | - |
dc.contributor.author | Yang, EM | - |
dc.contributor.author | Lee, HN | - |
dc.contributor.author | Cho, BY | - |
dc.contributor.author | Ye, YM | - |
dc.contributor.author | Park, HS | - |
dc.date.accessioned | 2010-12-22T07:09:34Z | - |
dc.date.available | 2010-12-22T07:09:34Z | - |
dc.date.issued | 2009 | - |
dc.identifier.issn | 0105-4538 | - |
dc.identifier.uri | http://repository.ajou.ac.kr/handle/201003/761 | - |
dc.description.abstract | BACKGROUND: It has been known that interleukin (IL)-10 promoter polymorphisms at -1082A/G, -819T/C and -592A/C, may influence IL-10 expression and associate with asthma. Interleukin-10 facilitates the regulatory function of transforming growth factor (TGF)-beta. The goal of this study was to investigate a gene-gene interaction between IL-10 and TGF-beta1 polymorphisms in Korean asthmatics with aspirin hypersensitivity.
METHODS: Single-nucleotide polymorphism genotyping of IL-10 and TGF-beta1 genes was performed and the functional effect of the IL-10 polymorphisms was analysed applying a luciferase reporter assay and an electrophoretic mobility shift assay. RESULTS: Among the patients with asthma, polymorphism at -1082A/G was significantly associated with the phenotype of aspirin-intolerant asthma, AIA (P = 0.007, P(c) = 0.021). Moreover, a synergistic effect between the TGF-beta1-509C/T and IL-10-1082A/G polymorphisms on the phenotype of AIA was noted; when stratified by the presence of rhinosinusitis, the frequency of rare alleles (the CT or TT genotype of TGF-beta1-509C/T and AG or GG genotype of IL-10-1082A/G) was significantly higher in the patients with AIA (15.2%) when compared with those with ATA (6.3%, P = 0.031; odds ratio 4.111; 95% confidence interval 1.504-11.235). In an in vitro functional assay, the -1082G reporter plasmid exhibited significantly greater promoter activity when compared with the -1082A construct in Jurkat T cells (P = 0.011). Moreover, we found that the transcription factor Myc-associated zinc-finger protein preferentially bound the -1082G allele. CONCLUSION: Our results suggest that IL-10 promoter polymorphisms contribute to the development of AIA and that rhinosinusitis may interact genetically with TGF-beta1. | - |
dc.language.iso | en | - |
dc.subject.MESH | Adult | - |
dc.subject.MESH | Alleles | - |
dc.subject.MESH | Aspirin | - |
dc.subject.MESH | Asthma | - |
dc.subject.MESH | Drug Hypersensitivity | - |
dc.subject.MESH | Epistasis, Genetic | - |
dc.subject.MESH | Female | - |
dc.subject.MESH | Gene Frequency | - |
dc.subject.MESH | Genetic Predisposition to Disease | - |
dc.subject.MESH | Humans | - |
dc.subject.MESH | Interleukin-10 | - |
dc.subject.MESH | Jurkat Cells | - |
dc.subject.MESH | Korea | - |
dc.subject.MESH | Male | - |
dc.subject.MESH | Polymorphism, Genetic | - |
dc.subject.MESH | Promoter Regions, Genetic | - |
dc.subject.MESH | Rhinitis, Allergic, Perennial | - |
dc.subject.MESH | Sinusitis | - |
dc.subject.MESH | Transforming Growth Factor beta1 | - |
dc.title | Combined effect of IL-10 and TGF-beta1 promoter polymorphisms as a risk factor for aspirin-intolerant asthma and rhinosinusitis. | - |
dc.type | Article | - |
dc.identifier.pmid | 19222424 | - |
dc.identifier.url | http://onlinelibrary.wiley.com/resolve/openurl?genre=article&sid=nlm:pubmed&issn=0105-4538&date=2009&volume=64&issue=8&spage=1221 | - |
dc.contributor.affiliatedAuthor | 예, 영민 | - |
dc.contributor.affiliatedAuthor | 박, 해심 | - |
dc.type.local | Journal Papers | - |
dc.identifier.doi | 10.1111/j.1398-9995.2009.01989.x | - |
dc.citation.title | Allergy | - |
dc.citation.volume | 64 | - |
dc.citation.number | 8 | - |
dc.citation.date | 2009 | - |
dc.citation.startPage | 1221 | - |
dc.citation.endPage | 1225 | - |
dc.identifier.bibliographicCitation | Allergy, 64(8). : 1221-1225, 2009 | - |
dc.identifier.eissn | 1398-9995 | - |
dc.relation.journalid | J001054538 | - |
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