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Hepatic differentiation of cord blood-derived multipotent progenitor cells (MPCs) in vitro.

Authors
Moon, YJ; Lee, MW; Yoon, HH; Yang, MS; Jang, IK; Lee, JE; Kim, HE; Eom, YW; Park, JS; Kim, HC; Kim, YJ; Lee, KH
Citation
Cell biology international, 32(10):1293-1301, 2008
Journal Title
Cell biology international
ISSN
1065-69951095-8355
Abstract
Umbilical cord blood (UCB) is a rich source of hematopoietic stem cells that possesses practical and ethical advantages. We previously reported a novel UCB-derived adult stem cells which we termed umbilical cord blood-derived multipotent progenitor cells' (MPCs). MPCs were capable of differentiating into functional neuronal cells. Under appropriate conditions lasting several days or weeks, we now show that the MPCs differentiate into hepatocyte-like cells in vitro; their properties were verified using reverse transcription-polymerase chain reaction (RT-PCR), Western blot, immunofluorescence, periodic acid-Schiff (PAS) staining of accumulated glycogen and an enzyme-linked immunosorbent assay (ELISA). We also found that hepatic differentiated cells expressed hepatocyte specific markers, such as albumin, hepatocyte nuclear factor (HNF)-1alpha, HNF4, cytokeratin (CK)-8, CK-18, tyrosine amino transferase (TAT), and CYP2B6. Moreover, albumin was secreted, which suggests that MPCs from UCB possess multi-differentiation potential and have the capacity to differentiate into functional cells of hepatic lineage in vitro.
MeSH terms
AdultBiological Markers/metabolismCell Differentiation/physiology*Cell ShapeCells, CulturedChildCulture Media, ConditionedFetal Blood/cytology*Fibroblast Growth Factor 4/metabolismHepatocytes/cytologyHepatocytes/physiology*HumansLiver/cytologyLiver/physiology*Multipotent Stem Cells/cytologyMultipotent Stem Cells/physiology*
DOI
10.1016/j.cellbi.2008.07.017
PMID
18703150
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Hematology-Oncology
AJOU Authors
박, 준성김, 효철
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