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New phytopharmaceutical agent CJ-20001 modulates stress-induced inflammatory infiltration into gastric mucosa

Authors
Yeo, M; Kim, DK; Cho, SW; Lee, SJ; Cho, IH; Song, GS; Moon, BS
Citation
Hepato-gastroenterology, 59(115):942-946, 2012
Journal Title
Hepato-gastroenterology
ISSN
0172-6390
Abstract
BACKGROUND/AIMS: CJ-20001 is a phytopharmaceutical agent and currently being investigated in a Phase II trial for the treatment of acute and chronic gastritis patients in Korea. In this study we addressed the protective effects of CJ-20001 against water immersion restraint stress (WIRS)-induced gastric injury in rats and studied the underlying mechanisms.



METHODOLOGY: To evaluate the protective effect of CJ-20001 on stress-induced gastric lesions, rats were exposed to water immersion restraint stress. Inflammatory infiltration into gastric mucosa was examined by immunohistochemistry and in vitro invasion assay. Expression of proinflammatory cytokines was detected with reverse transcription-polymerase chain reaction (RT-PCR).



RESULTS: Pretreatment with CJ-20001 dose-dependently attenuated the WIRS-induced gastric lesions as demonstrated by gross pathology and histology. WIRS increased infiltration of mast cells and macrophages into the gastric mucosa and submucosal layer, whereas the inflammatory infiltration was markedly inhibited by CJ-20001 administration. An in vitro cell invasion assay showed that treatment with CJ-20001 decreased the migration of macrophages. CJ-20001 suppressed the expression of proinflammatory cytokines, IL-18, IP-10 and GRO/KC, in lipopolysaccharides (LPS)-treated macrophages.



CONCLUSIONS: These data suggest that novel phytopharmaceutical agent CJ-20001 has the potent anti-inflammatory properties through inhibition of inflammatory infiltration in psycho-physiological stress-induced gastric injury.
MeSH terms
AnimalsAnti-Inflammatory Agents/*pharmacologyChemotaxis/drug effectsCytokines/genetics/metabolismCytoprotectionDisease Models, AnimalDose-Response Relationship, DrugGastric Mucosa/*drug effects/immunology/pathologyGastritis/etiology/genetics/immunology/pathology/*prevention & controlGastrointestinal Agents/*pharmacologyHumansImmersionImmunohistochemistryInflammation Mediators/metabolismLipopolysaccharides/pharmacologyMacrophages/drug effects/immunologyMaleMast Cells/drug effects/immunologyPlant Extracts/*pharmacologyPlant Preparations/*pharmacologyRNA, Messenger/metabolismRatsRats, Sprague-DawleyRestraint, PhysicalReverse Transcriptase Polymerase Chain ReactionStress, Psychological/complications/*drug therapy/etiology/genetics/immunology/pathologyU937 Cells
DOI
10.5754/hge11560
PMID
22024230
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Gastroenterology
AJOU Authors
조, 성원
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