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Identification of novel peptides that stimulate human neutrophils

Authors
Bae, GH; Lee, HY; Jung, YS; Shim, JW; Kim, SD; Baek, SH; Kwon, JY; Park, JS; Bae, YS
Citation
Experimental & molecular medicine, 44(2):130-137, 2012
Journal Title
Experimental & molecular medicine
ISSN
1226-36132092-6413
Abstract
Neutrophils play a key role in innate immunity, and the identification of new stimuli that stimulate neutrophil activity is a very important issue. In this study, we identified three novel peptides by screening a synthetic hexapeptide combinatorial library. The identified peptides GMMWAI, MMHWAM, and MMHWFM caused an increase in intracellular Ca2+ in a concentration-dependent manner via phospholipase C activity in human neutrophils. The three peptides acted specifically on neutrophils and monocytes and not on other non-leukocytic cells. As a physiological characteristic of the peptides, we observed that the three peptides induced chemotactic migration of neutrophils as well as stimulated superoxide anion production. Studying receptor specificity, we observed that two of the peptides (GMMWAI and MMHWFM) acted on formyl peptide receptor (FPR)1 while the other peptide (MMHWAM) acted on FPR2. Since the three novel peptides were specific agonists for FPR1 or FPR2, they might be useful tools to study FPR1- or FPR2-mediated immune response and signaling.
MeSH terms
AnimalsCalcium/metabolismCell LineCells, CulturedChemotaxis, Leukocyte/drug effectsHumansMiceNIH 3T3 CellsNeutrophils/*cytology/*drug effectsPC12 CellsPeptides/*pharmacologyRatsReceptors, Formyl Peptide/agonists
DOI
10.3858/emm.2012.44.2.008
PMID
22089089
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Hematology-Oncology
AJOU Authors
박, 준성
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