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Quantum dot-based screening system for discovery of g protein-coupled receptor agonists

Authors
Lee, J; Kwon, YJ; Choi, Y; Kim, HC; Kim, K; Kim, J; Park, S; Song, R
Citation
Chembiochem : a European journal of chemical biology, 13(10):1503-1508, 2012
Journal Title
Chembiochem : a European journal of chemical biology
ISSN
1439-42271439-7633
Abstract
Cellular imaging has emerged as an important tool to unravel biological complexity and to accelerate the drug-discovery process, including cell-based screening, target identification, and mechanism of action studies. Recently, semiconductor nanoparticles known as quantum dots (QDs) have attracted great interest in cellular imaging applications due to their unique photophysical properties such as size, tunable optical property, multiplexing capability, and photostability. Herein, we show that QDs can also be applied to assay development and eventually to high-throughput/content screening (HTS/HCS) for drug discovery. We have synthesized QDs modified with PEG and primary antibodies to be used as fluorescent probes for a cell-based HTS system. The G protein-coupled receptor (GPCR) family is known to be involved in most major diseases. We therefore constructed human osteosarcoma (U2OS) cells that specifically overexpress two types of differently tagged GPCRs: influenza hemagglutinin (HA) peptide-tagged κ-opioid receptors (κ-ORs) and GFP-tagged A3 adenosine receptors (A3AR). In this study, we have demonstrated that 1) anti-HA antibody-conjugated QDs could specifically label HA-tagged κ-ORs, 2) subsequent treatment of QD-tagged GPCR agonists allowed agonist-induced translocation to be monitored in real time, 3) excellent emission spectral properties of QD permitted the simultaneous detection of two GPCRs in one cell, and 4) the robust imaging capabilities of the QD-antibody conjugates could lead to reproducible quantitative data from high-content cellular images. These results suggest that the present QD-based GPCR inhibitor screening system can be a promising platform for further drug screening applications.
MeSH terms
Antibodies/chemistry/immunologyCell Line, TumorFluorescent Dyes/chemistryGreen Fluorescent Proteins/genetics/metabolismHemagglutinins/immunologyHumansMicroscopy, ConfocalPolyethylene Glycols/chemistry*Quantum DotsReceptor, Adenosine A3/genetics/metabolismReceptors, G-Protein-Coupled/*agonists/metabolismReceptors, Opioid, kappa/agonists/metabolismRecombinant Fusion Proteins/genetics/metabolism
DOI
10.1002/cbic.201200128
PMID
22692979
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
AJOU Authors
박, 선
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