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Induction of interleukin-8 by Naegleria fowleri lysates requires activation of extracellular signal-regulated kinase in human astroglial cells.

Authors
Kim, JH; Sohn, HJ; Lee, SH; Kwon, D; Shin, HJ
Citation
Parasitology research, 111(2):587-592, 2012
Journal Title
Parasitology research
ISSN
0932-01131432-1955
Abstract
Naegleria fowleri is a pathogenic free-living amoeba which causes primary amoebic meningoencephalitis in humans and experimental animals. To investigate the mechanisms of such inflammatory diseases, potential chemokine gene activation in human astroglial cells was investigated following treatment with N. fowleri lysates. We demonstrated that N. fowleri are potent inducers for the expression of interleukin-8 (IL-8) genes in human astroglial cells which was preceded by activation of extracellular signal-regulated kinase (ERK). In addition, N. fowleri lysates induces the DNA binding activity of activator protein-1 (AP-1), an important transcription factor for IL-8 induction. The specific mitogen-activated protein kinase kinase/ERK inhibitor, U0126, blocks N. fowleri-mediated AP-1 activation and subsequent IL-8 induction. N. fowleri-induced IL-8 expression requires activation of ERK in human astroglial cells. These findings indicate that treatment of N. fowleri on human astroglial cells leads to the activation of AP-1 and subsequent expression of IL-8 which are dependent on ERK activation. These results may help understand the N. fowleri-mediated upregulation of chemokine and cytokine expression in the astroglial cells.
MeSH terms
AnimalsAstrocytes/*drug effects/*metabolismButadienesCell LineEnzyme-Linked Immunosorbent AssayExtracellular Signal-Regulated MAP Kinases/genetics/*metabolismGene Expression Regulation/*drug effectsHumansInterleukin-8/genetics/*metabolismNaegleria fowleri/*chemistryNitrilesTranscription Factor AP-1/genetics/metabolism
DOI
10.1007/s00436-012-2872-3
PMID
22411631
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
AJOU Authors
김, 종현신, 호준
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