179 525

Cited 5 times in

An anti-nucleic acid antibody delivers antigen to the cross-presentation pathway in dendritic cells and potentiates therapeutic antitumor effects

DC Field Value Language
dc.contributor.authorPham, CD-
dc.contributor.authorWoo, MY-
dc.contributor.authorKim, YS-
dc.contributor.authorPark, S-
dc.contributor.authorKwon, MH-
dc.date.accessioned2013-04-23T06:16:51Z-
dc.date.available2013-04-23T06:16:51Z-
dc.date.issued2012-
dc.identifier.issn0022-1767-
dc.identifier.urihttp://repository.ajou.ac.kr/handle/201003/7884-
dc.description.abstractCross-presentation is important for initiating CTL responses against tumors. Delivery of exogenous Ags to the cross-presentation pathway in dendritic cells (DCs), using a number of different carriers, has been attempted to further understand the mechanisms underlying cross-presentation and to develop therapeutic tumor vaccines. The present study reports a new antigenic carrier molecule: a single-chain V region fragment (scFv) of a nucleic acid-hydrolyzing Ab, 3D8. A fusion protein comprising 3D8 scFv and the CTL epitope OVA(250-264) (chicken OVA aa 250-264) was internalized by DC2.4 DCs and processed via a proteasome-dependent, brefeldin- and cycloheximide-sensitive, chloroquine- and primaquine-insensitive pathway, resulting in loading of the CTL epitope onto H-2K(b). In vivo cross-presentation and cross-priming were efficient, even without adjuvant; injection of mice with 3D8 scFv-OVA(250-264) induced cross-presentation of the CTL epitope by draining lymph node CD11c(+) B7.1(+) MHC class II(high) DCs, elicited a CTL response, and suppressed the growth of tumors expressing the OVA epitope. This report shows that an anti-nucleic acid Ab is used to deliver exogenous Ag to the cross-presentation pathway and inhibit in vivo tumor growth.-
dc.language.isoen-
dc.subject.MESHAnimals-
dc.subject.MESHAntibodies, Antinuclear/genetics/*metabolism-
dc.subject.MESHAntigens, Neoplasm/genetics/immunology/*metabolism-
dc.subject.MESHCell Line, Tumor-
dc.subject.MESHChickens-
dc.subject.MESHCoculture Techniques-
dc.subject.MESHCross-Priming/genetics/*immunology-
dc.subject.MESHDendritic Cells/*immunology/metabolism/pathology-
dc.subject.MESHEpitopes, T-Lymphocyte/genetics/metabolism-
dc.subject.MESHMelanoma, Experimental/genetics/*immunology/*therapy-
dc.subject.MESHMice-
dc.subject.MESHMice, Inbred C57BL-
dc.subject.MESHProtein Transport/immunology-
dc.subject.MESHRecombinant Fusion Proteins/genetics/metabolism-
dc.subject.MESHSingle-Chain Antibodies/*metabolism/therapeutic use-
dc.subject.MESHTumor Escape/genetics/*immunology-
dc.titleAn anti-nucleic acid antibody delivers antigen to the cross-presentation pathway in dendritic cells and potentiates therapeutic antitumor effects-
dc.typeArticle-
dc.identifier.pmid23152565-
dc.identifier.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3516678/-
dc.contributor.affiliatedAuthor박, 선-
dc.contributor.affiliatedAuthor권, 명희-
dc.type.localJournal Papers-
dc.identifier.doi10.4049/jimmunol.1200804-
dc.citation.titleJournal of immunology (Baltimore, Md. : 1950)-
dc.citation.volume189-
dc.citation.number12-
dc.citation.date2012-
dc.citation.startPage5755-
dc.citation.endPage5763-
dc.identifier.bibliographicCitationJournal of immunology (Baltimore, Md. : 1950), 189(12):5755-5763, 2012-
dc.identifier.eissn1550-6606-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Microbiology
Files in This Item:
23152565.pdfDownload

qrcode

해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

Browse