111 547

Cited 17 times in

A MAP kinase pathway is implicated in the pseudohyphal induction by hydrogen peroxide in Candica albicans.

DC Field Value Language
dc.contributor.authorSrinivasa, K-
dc.contributor.authorKim, J-
dc.contributor.authorYee, S-
dc.contributor.authorKim, W-
dc.contributor.authorChoi, W-
dc.description.abstractHydrogen peroxide (H(2)O(2)) functions as a ubiquitous intracellular messenger besides as an oxidative stress molecule. This dual role is based on the distinct cellular responses against different concentrations of H(2)O(2). Previously, we demonstrated that both low (> 1 mM) and high (4-10 mM) doses of exogenous H(2)O(2) induce filamentous growth with distinct cell morphology and growth rate in Candida albicans, suggesting the different transcription response. In this study, we revealed that the sub-toxic and toxic levels of H(2)O(2) indeed induced pseudohyphae, but not true hyphae. Supporting this, several hyphae-specific genes that are expressed in true hyphae induced by serum were not detected in either sub-toxic or toxic H(2)O(2) condition. A DNA microarray analysis was conducted to reveal the transcription profiles in cells treated with sub-toxic and toxic conditions of H(2)O(2). Under the sub-toxic condition, a small number of genes involved in cell proliferation and metabolism were up-regulated, whereas a large number of genes were up-regulated in the toxic condition where the genes required for growth and proliferation were selectively restricted. For pseudohyphal induction by sub-toxic H(2)O(2), Cek1 MAPK activating the transcription factor Cph1 was shown to be important. The absence of expression of several hyphae-specific genes known to be downstream targets of Cph1-signaling pathway for true hyphae formation suggests that the Cek1-mediated signaling pathway is not solely responsible for pseudohyphal formation by subtoxic H(2)O(2) and, but instead, complex networking pathway may exists by the activation of different regulators.-
dc.subject.MESHCandida albicans/enzymology/genetics/*growth & development-
dc.subject.MESHCell Proliferation-
dc.subject.MESHFungal Proteins/genetics/*metabolism-
dc.subject.MESHGene Expression Profiling-
dc.subject.MESHHydrogen Peroxide/chemistry/pharmacology-
dc.subject.MESHHyphae/enzymology/genetics/*growth & development-
dc.subject.MESH*MAP Kinase Signaling System-
dc.subject.MESHMitogen-Activated Protein Kinase 3/genetics/*metabolism-
dc.subject.MESHOligonucleotide Array Sequence Analysis-
dc.subject.MESHOxidative Stress/genetics-
dc.subject.MESHTranscription Factors/genetics/*metabolism-
dc.titleA MAP kinase pathway is implicated in the pseudohyphal induction by hydrogen peroxide in Candica albicans.-
dc.contributor.affiliatedAuthor김, 완기-
dc.type.localJournal Papers-
dc.citation.titleMolecules and cells-
dc.identifier.bibliographicCitationMolecules and cells, 33(2):183-193, 2012-
Appears in Collections:
Journal Papers > School of Medicine / Graduate School of Medicine > Pharmacology
Files in This Item:
There are no files associated with this item.


해당 아이템을 이메일로 공유하기 원하시면 인증을 거치시기 바랍니다.

Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.